Human/Mouse/Rat HNF-3 beta /FoxA2 Antibody

Recombinant Monoclonal Antibody.
  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human HNF‑3 beta /FoxA2 in Western blots.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 2141A
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human/Mouse/Rat HNF‑3 beta /FoxA2 synthetic peptide
    Accession # Q9Y261
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunohistochemistry
    3-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human HNF‑3 beta /FoxA2 by Western Blot. Western blot shows lysates of human lung tissue and human pancreas tissue. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human/Mouse/Rat HNF‑3 beta /FoxA2 Monoclonal Antibody (Catalog # MAB2400) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HNF‑3 beta /FoxA2 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of HNF‑3 beta /FoxA2 in Human HepG2 Cells by Flow Cytometry. Human HepG2 cell line was stained with Rabbit Anti-Human/Mouse/Rat HNF-3 beta/FoxA2 Monoclonal Antibody (Catalog # MAB2400, filled histogram) or Normal Rabbit IgG Control (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).
Detection of HNF-3 beta /FoxA2 in Human iPS Cells differentiated to Mesoderm by Flow Cytometry. Human iPS cells differentiated to Mesoderm (Catalog # SC030B) were stained with Rabbit Anti-Human/Mouse/Rat HNF-3 beta/FoxA2 Monoclonal Antibody (Catalog # MAB2400, filled histogram) or Normal Rabbit IgG Control (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).
Immunohistochemistry
HNF‑3 beta /FoxA2 in Human Liver Cancer Tissue. HNF‑3 beta /FoxA2 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Rabbit Anti-Human/Mouse/Rat HNF‑3 beta /FoxA2 Monoclonal Antibody (Catalog # MAB2400) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HNF-3 beta/FoxA2
HNF-3 beta, also known as FoxA2, is a member of the forkhead class of DNA-binding proteins. It is a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. Similar family members play roles in the differentiation of the pancreas and liver.
  • Long Name:
    Hepatocyte Nuclear Factor-3, beta
  • Entrez Gene IDs:
    3170 (Human)
  • Alternate Names:
    forkhead box A2; Forkhead box protein A2; FoxA2; hepatic nuclear factor-3-beta; hepatocyte nuclear factor 3, beta; hepatocyte nuclear factor 3-beta; HNF3 beta; HNF-3 beta; HNF-3B; HNF-3-beta; HNF3BTCF-3B; MGC19807; TCF3B; Transcription factor 3B
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