Detection of Human, Mouse, and Rat HSPB8 by Western Blot.
Western blot shows lysates of human, mouse, and rat heart tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat HSPB8 Monoclonal Antibody (Catalog # MAB4987) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSPB8 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Heat shock protein beta-8 (HSPB8, also HSP22 or CRYAC) is a 22‑25 kDa member of the small HSP (HSP20) family of proteins. It is expressed in muscle (smooth, skeletal and cardiac), and serves as a molecular chaperone. Human HSPB8 contains one alpha -crystalline domain (aa 93‑170) that mediates protein-protein interaction, and N- plus C-terminal flanking sequences that generate homodimers, homooligomers, and heterodimers with HSP27 and HSPB7. Phosphorylation at Ser57 blocks HSPB8 chaperone activity. Human HSPB8 shares 94% aa identity with both mouse and canine HSPB8. Deficiencies in HSPB8 are involved in distal motor neuropathy type 11A.
Heat Shock Protein beta 8
Entrez Gene IDs:
26353 (Human); 80888 (Mouse); 113906 (Rat)
Alpha-crystallin C chain; CMT2L; CRYAC; DHMN2; E2IG1; E2IG1E2-induced gene 1 protein; H11; H11DHMN2; heat shock 22kDa protein 8; heat shock 27kDa protein 8; heat shock protein beta-8; HMN2; HMN2A; HSP22; HSP22CMT2L; HSPB8; Protein kinase H11; Small stress protein-like protein HSP22
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