Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Rat

Applications

Validated:

Western Blot

Cited:

Western Blot, Immunocytochemistry, Co-Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 520817
View all HSPB8 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HSPB8
Ala2-Thr196
Accession # Q9UJY1

Specificity

Detects human, mouse, and rat HSPB8 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat HSPB8 Antibody

Detection of Human, Mouse, and Rat HSPB8 antibody by Western Blot.

Detection of Human, Mouse, and Rat HSPB8 by Western Blot.

Western blot shows lysates of human, mouse, and rat heart tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat HSPB8 Monoclonal Antibody (Catalog # MAB4987) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSPB8 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human/Mouse/Rat HSPB8 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: Human, mouse, and rat heart tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HSPB8

Heat shock protein beta-8 (HSPB8, also HSP22 or CRYAC) is a 22‑25 kDa member of the small HSP (HSP20) family of proteins. It is expressed in muscle (smooth, skeletal and cardiac), and serves as a molecular chaperone. Human HSPB8 contains one alpha -crystalline domain (aa 93‑170) that mediates protein-protein interaction, and N- plus C-terminal flanking sequences that generate homodimers, homooligomers, and heterodimers with HSP27 and HSPB7. Phosphorylation at Ser57 blocks HSPB8 chaperone activity. Human HSPB8 shares 94% aa identity with both mouse and canine HSPB8. Deficiencies in HSPB8 are involved in distal motor neuropathy type 11A.

Long Name

Heat Shock Protein beta 8

Alternate Names

CMT2L, CRYAC, DHMN2, E2IG1, H11, HMN2A, HSP22

Entrez Gene IDs

26353 (Human); 80888 (Mouse); 113906 (Rat)

Gene Symbol

HSPB8

UniProt

Q9UJY1 (Human)

Additional HSPB8 Products

Product Documents for Human/Mouse/Rat HSPB8 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat HSPB8 Antibody

For research use only

Citations for Human/Mouse/Rat HSPB8 Antibody

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