Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 499526
View all ILKAP/PP2C delta Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ILKAP
Met1-His392
Accession # Q9H0C8

Specificity

Detects endogenous human, mouse and rat ILKAP in Western blots. In Western blots, this antibody does not cross-react with recombinant human PP2C alpha, PP2C beta or PPC2 gamma.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat ILKAP/PP2C delta Antibody

Detection of Human ILKAP/PP2Cd antibody by Western Blot.

Detection of Human ILKAP/PP2C delta by Western Blot.

Western blot shows lysates of PC-3 human prostate cancer cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat ILKAP/PP2Cd Monoclonal Antibody (Catalog # MAB4554) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ILKAP/PP2Cd at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat ILKAP/PP2C delta Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: PC-3 human prostate cancer cell line and HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ILKAP/PP2C delta

Integrin Linked Kinase 1 Associated Phosphatase (ILKAP), also called PP2C delta, is a serine/threonine phosphatase that is stimulated by manganese but inhibited by magnesium ions and is insensitive to the phosphatase inhibitor okadaic acid. It binds to ILK1 and dephosphorylates it, decreasing the stimulation of the GSK-3 beta pathway by cell adhesion without affecting Akt signaling. Transfection studies have shown that ILKAP activity inhibits cell cycle progression from the G1 to S phase. ILKAP is expressed in many tissues but is highest in liver, and skeletal and cardiac muscle. Its gene is found in a region of chromosome 2 that is frequently deleted in oral cancers.

Long Name

Integrin-Linked Kinase-Associated Serine/Threonine Phosphatase 2C

Alternate Names

PP2C delta

Entrez Gene IDs

80895 (Human); 67444 (Mouse); 64538 (Rat)

Gene Symbol

ILKAP

UniProt

Q9H0C8

Additional ILKAP/PP2C delta Products

Product Documents for Human/Mouse/Rat ILKAP/PP2C delta Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat ILKAP/PP2C delta Antibody

For research use only

Related Research Areas

Epigenetics
Phosphatases and Regulators

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