Human/Mouse/Rat ILKAP/PP2C delta Antibody

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Detection of Human ILKAP/PP2Cδ by Western Blot.
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Human/Mouse/Rat ILKAP/PP2C delta Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects endogenous human, mouse and rat ILKAP in Western blots. In Western blots, this antibody does not cross-react with recombinant human PP2C alpha, PP2C beta or PPC2 gamma.
Monoclonal Mouse IgG2B Clone # 499526
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human ILKAP
Accession # Q9H0C8
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human ILKAP/PP2Cd antibody by Western Blot. View Larger

Detection of Human ILKAP/PP2Cδ by Western Blot. Western blot shows lysates of PC-3 human prostate cancer cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat ILKAP/PP2Cd Monoclonal Antibody (Catalog # MAB4554) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ILKAP/PP2Cd at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ILKAP/PP2C delta

Integrin Linked Kinase 1 Associated Phosphatase (ILKAP), also called PP2Cδ, is a serine/threonine phosphatase that is stimulated by manganese but inhibited by magnesium ions and is insensitive to the phosphatase inhibitor okadaic acid. It binds to ILK1 and dephosphorylates it, decreasing the stimulation of the GSK-3 beta pathway by cell adhesion without affecting Akt signaling. Transfection studies have shown that ILKAP activity inhibits cell cycle progression from the G1 to S phase. ILKAP is expressed in many tissues but is highest in liver, and skeletal and cardiac muscle. Its gene is found in a region of chromosome 2 that is frequently deleted in oral cancers.

Long Name
Integrin-Linked Kinase-Associated Serine/Threonine Phosphatase 2C
Entrez Gene IDs
80895 (Human); 67444 (Mouse); 64538 (Rat)
Alternate Names
DKFZp434J2031; EC; FLJ10181; ILKAP; integrin-linked kinase-associated serine/threonine phosphatase 2C; integrin-linked kinase-associated serine/threonine phosphatase; MGC4846; PP2C delta; PP2C-DELTA; protein phosphatase 2c, delta isozyme

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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