Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, rat, and mouse IMP Dehydrogenase 1/IMPDH1 in direct ELISAs and Western blots. In direct ELISAs, less than
    5% cross-reactivity with recombinant mouse IMPDH2 is observed.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf21 derived recombinaint human IMP Dehydrogenase 1/IMPDH1
    Met1-Tyr563 (predicted)
    Accession # P20839
  • Formulation
    Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution
    See below
  • Immunocytochemistry
    1:200 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse IMP Dehydrogenase 1/IMPDH1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and BaF3 mouse pro-B cell line. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8554) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for IMP Dehydrogenase 1/IMPDH1 at approximately 55-57 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
IMP Dehydrogenase 1/IMPDH1 in HeLa Human Cervical Epithelial Carcinoma Cell Line. IMP Dehydrogenase 1/IMPDH1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8554) at a 1:200 dilution for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm and nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C, as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after opening.
    • 6 months, -20 to -70 °C under sterile conditions after opening.
Background: IMP Dehydrogenase 1/IMPDH1
The enzyme IMPDH1 (Inosine-5'-monophosphate dehydrogenase 1) catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.The enzyme is 514 aminoacids long, with a predicted molecular weight of 55 kDa.
  • Long Name:
    Inosine 5'-Monophosphate Dehydrogenase 1
  • Entrez Gene IDs:
    3614 (Human); 23917 (Mouse); 362329 (Rat)
  • Alternate Names:
    DKFZp781N0678; EC 1.1.1.205; IMP (inosine 5'-monophosphate) dehydrogenase 1; IMP (inosine monophosphate) dehydrogenase 1; IMP Dehydrogenase 1; IMPD 1; IMPD; IMPD1; IMPDH 1; IMPDH1; IMPDH-I; inosine-5'-monophosphate dehydrogenase 1; LCA11; LCA11RP10; retinitis pigmentosa 10 (autosomal dominant); RP10; sWSS2608
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