Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antibody
R&D Systems | Catalog # AF8554
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf21 derived recombinaint human IMP Dehydrogenase 1/IMPDH1
Met1-Tyr563 (predicted)
Accession # P20839
Met1-Tyr563 (predicted)
Accession # P20839
Specificity
Detects human, rat, and mouse IMP Dehydrogenase 1/IMPDH1 in direct ELISAs and Western blots. In direct ELISAs, less than
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antibody
Detection of Human and Mouse IMP Dehydrogenase 1/IMPDH1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and BaF3 mouse pro-B cell line. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8554) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for IMP Dehydrogenase 1/IMPDH1 at approximately 55-57 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
IMP Dehydrogenase 1/IMPDH1 in HeLa Human Cervical Epithelial Carcinoma Cell Line.
IMP Dehydrogenase 1/IMPDH1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8554) at a 1:200 dilution for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm and nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
IMP Dehydrogenase 1/IMPDH1 in C2C12 Mouse Cell Line.
IMP Dehydrogenase 1/IMPDH1 was detected in immersion fixed C2C12 mouse myoblast cell line using Rabbit Anti-Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8554) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Human IMP Dehydrogenase 1/IMPDH1 by Simple WesternTM.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for IMP Dehydrogenase 1/IMPDH1 at approximately 56 kDa (as indicated) using 2 µg/mL of Rabbit Anti-Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8554). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antibody
Application
Recommended Usage
Immunocytochemistry
1:200 dilution
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line
Simple Western
2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line
Western Blot
1:1000 dilution
Sample: HeLa human cervical epithelial carcinoma cell line and BaF3 mouse pro-B cell line
Sample: HeLa human cervical epithelial carcinoma cell line and BaF3 mouse pro-B cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C, as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
Background: IMP Dehydrogenase 1/IMPDH1
Long Name
Inosine 5'-Monophosphate Dehydrogenase 1
Alternate Names
IMPD1, IMPDH1, LCA11, RP10
Gene Symbol
IMPDH1
UniProt
Additional IMP Dehydrogenase 1/IMPDH1 Products
Product Documents for Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antibody
* Contains 0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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