Human/Mouse/Rat Lipocalin-2/NGAL Antibody

Catalog # Availability Size / Price Qty
AF1757
AF1757-SP
Detection of Human Lipocalin‑2/NGAL by Western Blot.
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Product Details
Citations (9)
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Human/Mouse/Rat Lipocalin-2/NGAL Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human Lipocalin-2/NGAL in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived Recombinant Human Lipocalin‑2/NGAL
Gln21-Gly198
Accession # P80188
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Lipocalin‑2/NGAL by Western Blot. View Larger

Detection of Human Lipocalin‑2/NGAL by Western Blot. Western blot shows lysates of Capan‑1 human pancreatic adenocarcinoma cell line and human pancreas tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1757) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Lipocalin‑2/NGAL at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection  of Mouse and Rat Lipocalin‑2/NGAL by Western Blot. View Larger

Detection of Mouse and Rat Lipocalin‑2/NGAL by Western Blot. Western blot shows lysates of mouse and rat bone marrow. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1757) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Lipocalin‑2/NGAL at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Lipocalin‑2/NGAL in Human Pancreatic Cancer Tissue. View Larger

Lipocalin‑2/NGAL in Human Pancreatic Cancer Tissue. Lipocalin‑2/NGAL was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Goat Anti-Human/Mouse/
Rat Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1757) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter­stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Lipocalin‑2/NGAL in Human Pancreatic Cancer Tissue. View Larger

Lipocalin‑2/NGAL in Human Pancreatic Cancer Tissue. Lipocalin‑2/NGAL was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Goat Anti-Human/Mouse/Rat Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1757) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human Lipocalin‑2/NGAL by Simple WesternTM. View Larger

Detection of Human Lipocalin‑2/NGAL by Simple WesternTM. Simple Western lane view shows lysates of Capan‑1 human pancreatic adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Lipocalin‑2/NGAL at approximately 34 kDa (as indicated) using 2 µg/mL of Goat Anti-Human/Mouse/Rat Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1757) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lipocalin-2/NGAL

Members of Lipocalin family share a highly conserved fold with an eight-stranded antiparallel beta barrel, and act as a transporters, carrying small molecules to specific cells. Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules. It is a 25 kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human neutrophil gelatinases (MMP-9). Its expression has been observed in most tissues normally exposed to microorganism, and its synthesis is induced in epithelial cells during inflammation. Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis. Studies indicate that Lipocalin-2 binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (Kd = 0.41 nM). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less Kd values (7.9 nM). Secretion of Lipocalin‑2 in immune cells increases by stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron. Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway.

Long Name
Neutrophil Gelatinase-associated Lipocalin
Entrez Gene IDs
3934 (Human); 16819 (Mouse); 170496 (Rat); 102137149 (Cynomolgus Monkey)
Alternate Names
24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin

Product Datasheets

Citations for Human/Mouse/Rat Lipocalin-2/NGAL Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Unexpected kidney-restricted role for IL-17 receptor signaling in defense against systemic Candida albicans infection
    Authors: K Ramani, CV Jawale, AH Verma, BM Coleman, JK Kolls, PS Biswas
    JCI Insight, 2018;3(9):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. Cilostazol attenuates kainic acid-induced hippocampal cell death
    Authors: YS Park, Z Jin, EA Jeong, CO Yi, JY Lee, IS Park, GS Roh
    Korean J. Physiol. Pharmacol., 2018;22(1):63-70.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Glycine mitigates renal oxidative stress by suppressing Nox4 expression in rats with streptozotocin-induced diabetes
    Authors: Z Wang, J Zhang, L Wang, W Li, L Chen, J Li, D Zhao, H Zhang, X Guo
    J. Pharmacol. Sci., 2018;137(4):387-394.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Metformin alleviates nickel-induced autophagy and apoptosis via inhibition of hexokinase-2, activating lipocalin-2, in human bronchial epithelial cells
    Authors: YT Kang, WC Hsu, CH Wu, IL Hsin, PR Wu, KT Yeh, JL Ko
    Oncotarget, 2017;8(62):105536-105552.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. The adipokine lipocalin-2 in the context of the osteoarthritic osteochondral junction
    Sci Rep, 2016;6(0):29243.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  6. Expression of uterine lipocalin 2 and its receptor during early- to mid-pregnancy period in mares
    Authors: Shingo Haneda
    J. Reprod. Dev, 2016;0(0):.
    Species: Equine
    Sample Types: Luminal Fluid
    Applications: Western Blot
  7. Lipocalin2 promotes invasion, tumorigenicity and gemcitabine resistance in pancreatic ductal adenocarcinoma.
    Authors: Leung, Lisa, Radulovich, Nikolina, Zhu, Chang-Qi, Organ, Shawna, Bandarchi, Bizhan, Pintilie, Melania, To, Christin, Panchal, Devang, Tsao, Ming Sou
    PLoS ONE, 2012;7(10):e46677.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Cellular settings mediating Src Substrate switching between focal adhesion kinase tyrosine 861 and CUB-domain-containing protein 1 (CDCP1) tyrosine 734.
    Authors: Wortmann A, He Y, Christensen ME, Linn M, Lumley JW, Pollock PM, Waterhouse NJ, Hooper JD
    J. Biol. Chem., 2011;286(49):42303-15.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Urinary neutrophil gelatinase-associated lipocalin levels reflect damage to glomeruli, proximal tubules, and distal nephrons.
    Authors: Kuwabara T, Mori K, Mukoyama M, Kasahara M, Yokoi H, Saito Y, Yoshioka T, Ogawa Y, Imamaki H, Kusakabe T, Ebihara K, Omata M, Satoh N, Sugawara A, Barasch J, Nakao K
    Kidney Int., 2009;75(3):285-94.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Reviews for Human/Mouse/Rat Lipocalin-2/NGAL Antibody

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Human/Mouse/Rat Lipocalin-2/NGAL Antibody
By Anonymous on 02/16/2018
Application: WB Sample Tested: HEK293T human embryonic kidney cell line Species: Human

Recombinant human LCN2 expressed in HEK-293T cells. Test during isolation and purification process.
1st band: 1st eluate (15ug)
2nd band: 2nd eluate (15ug)
3rd band: bead slurry
Dilution: 1:10000


Human/Mouse/Rat Lipocalin-2/NGAL Antibody
By Anonymous on 12/06/2017
Application: ELISA Sample Tested: EDTA Plasma Species: Human