Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 475417
View all LMW-PTP/ACP1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human LMW-PTP/ACP1
Ala2-His158
Accession # P24666

Specificity

Detects human, mouse, and rat LMW-PTP/ACP1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat LMW‑PTP/ACP1 Antibody

Detection of Human LMW-PTP/ACP1 antibody by Western Blot.

Detection of Human LMW-PTP/ACP1 by Western Blot.

Western blot shows lysates of CHP-100 human neuroblastoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat LMW-PTP/ACP1 Monoclonal Antibody (Catalog # MAB5075) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LMW-PTP/ACP1 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat LMW‑PTP/ACP1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: CHP‑100 human neuroblastoma cell line and HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LMW-PTP/ACP1

Low molecular weight protein tyrosine phosphatase (LMW-PTP), also known as Acid Phosphatase 1 (ACP1), is an 18 kDa cytosolic phosphatase that is unrelated to other tyrosine phosphatases, such as PTP1B. Tyrosine phosphorylation of LMW-PTP increases its activity 20-fold and affects its ability to dephosphorylate targets such as the PDGF receptor. Cancer cells overexpressing LMW-PTP have a higher proliferative rate and generate tumors that are larger than untransfected controls. Colon adenocarcinomas induced by dimethylhydrazine also have elevated levels of LMW-PTP, suggesting that this phosphatase may be a marker for oncogenic transformation. Human LMW-PTP shares 86% and 87% amino acid sequence identity with mouse and rat LMW-PTP, respectively.

Long Name

Low Molecular Weight Phosphotyrosine Protein Phosphotase

Alternate Names

ACP1, HAAP, LMWPTP

Entrez Gene IDs

52 (Human); 11431 (Mouse); 24161 (Rat)

Gene Symbol

ACP1

UniProt

P24666

Additional LMW-PTP/ACP1 Products

Product Documents for Human/Mouse/Rat LMW‑PTP/ACP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat LMW‑PTP/ACP1 Antibody

For research use only

Related Research Areas

Phosphatases and Regulators

Citations for Human/Mouse/Rat LMW‑PTP/ACP1 Antibody

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