Human/Mouse/Rat NRAGE Antibody

Catalog # Availability Size / Price Qty
AF3835
AF3835-SP
Detection of Human/Mouse/Rat NRAGE by Western Blot.
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Human/Mouse/Rat NRAGE Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects endogenous human, mouse and rat NRAGE in Western blot.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human NRAGE
Arg73-Asn254
Accession # Q9Y5V3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human/Mouse/Rat NRAGE by Western Blot. View Larger

Detection of Human/Mouse/Rat NRAGE by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, 293T human embryonic kidney cell line, A20 mouse B cell lymphoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat NRAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3835) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for NRAGE at approximately 110 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NRAGE

Neurotrophin receptor-interacting MAGE homolog (NRAGE, also known as MAGE-D1 or Dlxin-1) is a ubiquitously expressed cytosolic protein that is a member of the melanoma-associated antigen (MAGE) family. The 778 aa human NRAGE protein contains a segment with 22 repeats of a W(P/Q)xPxx motif, followed by a MAGE domain. In the brain, NRAGE interacts with p75NTR, blocks cell cycle progression and facilitates NGF-dependent apoptosis. Over amino acids 73‑254, human NRAGE shares 87% and 73% aa identity with mouse and rat NRAGE, respectively.

Long Name
Neurotrophin Receptor-interacting MAGE Homolog
Entrez Gene IDs
9500 (Human); 94275 (Mouse); 84469 (Rat)
Alternate Names
Dlxin-1; MAGE tumor antigen CCF; MAGED1; melanoma antigen family D, 1; melanoma-associated antigen D1; Neurotrophin receptor-interacting MAGE homolog; NRAGE MAGE-D1 antigen; NRAGE

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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