NRAGE Antibody

R&D Systems | Catalog # AF3835

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all NRAGE Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NRAGE
Arg73-Asn254
Accession # Q9Y5V3

Specificity

Detects endogenous human, mouse and rat NRAGE in Western blot.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NRAGE Antibody

Detection of Human/Mouse/Rat NRAGE antibody by Western Blot.

Detection of Human/Mouse/Rat NRAGE by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, 293T human embryonic kidney cell line, A20 mouse B cell lymphoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat NRAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3835) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for NRAGE at approximately 110 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Applications for NRAGE Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, 293T human embryonic kidney cell line, A20 mouse B cell lymphoma cell line, and L6 rat myoblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NRAGE

Neurotrophin receptor-interacting MAGE homolog (NRAGE, also known as MAGE-D1 or Dlxin-1) is a ubiquitously expressed cytosolic protein that is a member of the melanoma-associated antigen (MAGE) family. The 778 aa human NRAGE protein contains a segment with 22 repeats of a W(P/Q)xPxx motif, followed by a MAGE domain. In the brain, NRAGE interacts with p75NTR, blocks cell cycle progression and facilitates NGF-dependent apoptosis. Over amino acids 73‑254, human NRAGE shares 87% and 73% aa identity with mouse and rat NRAGE, respectively.

Long Name

Neurotrophin Receptor-interacting MAGE Homolog

Alternate Names

Dlxin-1, MAGED1

Entrez Gene IDs

9500 (Human); 94275 (Mouse); 84469 (Rat)

Gene Symbol

MAGED1

UniProt

Q9Y5V3 (Human)

Additional NRAGE Products

Product Documents for NRAGE Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for NRAGE Antibody

For research use only

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