p53 Antibody (184721)

R&D Systems | Catalog # MAB1355

R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Bacteria - Escherichia coli, Xenograft

Applications

Validated:

Western Blot, Immunocytochemistry, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot, Simple Western, Ubiquitination

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 184721
View all p53 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human p53
Asp7-Asp393
Accession # P04637

Specificity

Detects human, mouse, and rat p53.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for p53 Antibody (184721)

Detection of Human p53 antibody by Western Blot.

Detection of Human p53 by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line mock-treated (-) or treated (+) with 1 µM camptothecin (CPT) for 1 hour. Human p53 was immunoprecipitated using Mouse Anti-Human/Mouse/Rat p53 Monoclonal Antibody (Catalog # MAB1355). PVDF membrane was probed with HRP-conjugated Anti-Human/Mouse/Rat Polyclonal Antibody (Catalog # HAF1355). A specific band for p53 was detected at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
p53 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

p53 in HeLa Human Cell Line.

p53 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat p53 Monoclonal Antibody (Catalog # MAB1355) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Rat p53 by Western Blot

Detection of Rat p53 by Western Blot

Immunoblot analysis of kidney lysates.(A) Blot membranes were incubated with the antibodies against Nrf2, p53 and 8-oxoguanine glycosylase alpha (OGG1 alpha ). The mean integrated optical density is related to actin for Nrf2 (B), p53 (C) and mature 8-oxoguanine glycosylase alpha (D) levels. Results are given as a mean ± S.E.M. KD4, KD6, KD8 – groups of Eker rats (Tsc2+/−) treated with HFKD for four, six or eight mo., respectively; ST – Eker rats fed with a standard diet; LE ST – wild-type Long Evans rats treated with a standard diet; LE KD – wild-type Long Evans rats treated with a ketogenic diet similarly to the KD6 group. *P < 0.05, **p < 0.01, ***p < 0.001 as compared to ST unless otherwise stated (horizontal line) for ANOVA with a Fisher post hoc test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26892894), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for p53 Antibody (184721)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Immunoprecipitation

1-2 µg/500 µg cell lysate
Sample: CEM human T-lymphoblastoid cell line, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: p53 immunoprecipitated from lysates of MCF-7 human breast cancer cell line using anti-P53 monoclonal antibody???

Reviewed Applications

Read 1 review rated 5 using MAB1355 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: p53

The p53 tumor suppressor protein is a multi-functional transcription factor that regulates cellular decisions regarding proliferation, cell cycle checkpoints, and apoptosis. The importance of p53 is underscored by its mutation in over 50% of human cancers. Mice that lack one or both copies of p53 also showed an increased incidence of tumors, which makes the p53 deficient mouse a model system for studying cancer generation and progression.

Alternate Names

BCC7, LFS1, TP53, TRP53

Entrez Gene IDs

7157 (Human); 22059 (Mouse); 24842 (Rat)

Gene Symbol

TP53

UniProt

P04637

Additional p53 Products

Product Documents for p53 Antibody (184721)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for p53 Antibody (184721)

For research use only

Citations for p53 Antibody (184721)

Customer Reviews for p53 Antibody (184721) (1)

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  • Human/Mouse/Rat p53 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Cancer cell line
    Species: Human
    Verified Customer | Posted 10/13/2021
    p53 Antibody (184721) MAB1355

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Protocols

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