Human/Mouse/Rat p53 Antibody

(4 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat p53.
  • Source
    Monoclonal Mouse IgG2B Clone # 184721
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human p53
    Asp7-Asp393
    Accession # P04637
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Human p53 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line mock-treated (-) or treated (+) with 1 μM camptothecin (CPT) for 1 hour. Human p53 was immunoprecipitated using Mouse Anti-Human/Mouse/Rat p53 Monoclonal Antibody (Catalog # MAB1355). PVDF membrane was probed with HRP-conjugated Anti-Human/Mouse/Rat Polyclonal Antibody (Catalog # HAF1355). A specific band for p53 was detected at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

p53 in HeLa Human Cell Line. p53 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat p53 Monoclonal Antibody (Catalog # MAB1355) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p53

The p53 tumor suppressor protein is a multi-functional transcription factor that regulates cellular decisions regarding proliferation, cell cycle checkpoints, and apoptosis. The importance of p53 is underscored by its mutation in over 50% of human cancers. Mice that lack one or both copies of p53 also showed an increased incidence of tumors, which makes the p53 deficient mouse a model system for studying cancer generation and progression.

  • Entrez Gene IDs:
    7157 (Human); 22059 (Mouse); 24842 (Rat)
  • Alternate Names:
    Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Autophagy deficient keratinocytes display increased DNA damage, senescence and aberrant lipid composition after oxidative stress in vitro and in vivo
    Redox Biol, 2016;11(0):219-230.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. Long-term culture and expansion of primary human hepatocytes.
    Authors: Levy G, Bomze D, Heinz S, Ramachandran S, Noerenberg A, Cohen M, Shibolet O, Sklan E, Braspenning J, Nahmias Y
    Nat Biotechnol, 2015;33(12):1264-1271.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. DISC-mediated activation of caspase-2 in DNA damage-induced apoptosis.
    Authors: Olsson M, Vakifahmetoglu H, Abruzzo PM, Hogstrand K, Grandien A, Zhivotovsky B
    Oncogene, 2009;28(18):1949-59.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Functional connection between p53 and caspase-2 is essential for apoptosis induced by DNA damage.
    Authors: Vakifahmetoglu H, Olsson M, Orrenius S, Zhivotovsky B
    Oncogene, 2006;25(41):5683-92.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

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