Human/Mouse/Rat p53 Antibody

Catalog # Availability Size / Price Qty
Detection of Human p53 by Western Blot.
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Product Details
Citations (7)
Supplemental Products
Reviews (1)

Human/Mouse/Rat p53 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human, mouse, and rat p53.
Monoclonal Mouse IgG2B Clone # 184721
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human p53
Accession # P04637
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
1-2 µg/500 µg cell lysate
CEM human T-lymphoblastoid cell line, see our available Western blot detection antibodies
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human p53 antibody by Western Blot. View Larger

Detection of Human p53 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line mock-treated (-) or treated (+) with 1 µM camptothecin (CPT) for 1 hour. Human p53 was immunoprecipitated using Mouse Anti-Human/Mouse/Rat p53 Monoclonal Antibody (Catalog # MAB1355). PVDF membrane was probed with HRP-conjugated Anti-Human/Mouse/Rat Polyclonal Antibody (Catalog # HAF1355). A specific band for p53 was detected at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry p53 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

p53 in HeLa Human Cell Line. p53 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat p53 Monoclonal Antibody (Catalog # MAB1355) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p53

The p53 tumor suppressor protein is a multi-functional transcription factor that regulates cellular decisions regarding proliferation, cell cycle checkpoints, and apoptosis. The importance of p53 is underscored by its mutation in over 50% of human cancers. Mice that lack one or both copies of p53 also showed an increased incidence of tumors, which makes the p53 deficient mouse a model system for studying cancer generation and progression.

Entrez Gene IDs
7157 (Human); 22059 (Mouse); 24842 (Rat)
Alternate Names
Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53

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Citations for Human/Mouse/Rat p53 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Combined anticancer therapy with imidazoacridinone C-1305 and paclitaxel in human lung and colon cancer xenografts-Modulation of tumour angiogenesis
    Authors: M ?witalska, B Filip-Psur, M Milczarek, M Psurski, A Moszy?ska, AM D?browska, M Gawro?ska, K Krzymi?ski, M Bagi?ski, R Bartoszews, J Wietrzyk
    Oncogene, 2022;0(0):.
    Species: Xenograft
    Sample Types: Tissue Homogenates
    Applications: Capillary Immunoblot
  2. Upregulation of hepatic autophagy under nutritional ketosis
    Authors: D Li?kiewicz, A Li?kiewicz, M Grabowski, MM Nowacka-Ch, K Jab?o?ska, A Wojakowska, ? Marczak, JJ Barski, A Ma?ecki
    The Journal of nutritional biochemistry, 2021;0(0):108620.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. CSN7B defines a variant COP9 signalosome complex with distinct function in DNA damage response
    Authors: J Wang, D Dubiel, Y Wu, Y Cheng, DA Wolf, W Dubiel
    Cell Reports, 2021;34(4):108662.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Autophagy deficient keratinocytes display increased DNA damage, senescence and aberrant lipid composition after oxidative stress in vitro and in vivo
    Redox Biol, 2016;11(0):219-230.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Long-term culture and expansion of primary human hepatocytes.
    Authors: Levy G, Bomze D, Heinz S, Ramachandran S, Noerenberg A, Cohen M, Shibolet O, Sklan E, Braspenning J, Nahmias Y
    Nat Biotechnol, 2015;33(12):1264-1271.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. DISC-mediated activation of caspase-2 in DNA damage-induced apoptosis.
    Authors: Olsson M, Vakifahmetoglu H, Abruzzo PM, Hogstrand K, Grandien A, Zhivotovsky B
    Oncogene, 2009;28(18):1949-59.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Functional connection between p53 and caspase-2 is essential for apoptosis induced by DNA damage.
    Authors: Vakifahmetoglu H, Olsson M, Orrenius S, Zhivotovsky B
    Oncogene, 2006;25(41):5683-92.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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Reviews for Human/Mouse/Rat p53 Antibody

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Human/Mouse/Rat p53 Antibody
By Anonymous on 10/13/2021
Application: ICC/IF Sample Tested: Cancer cell line Species: Human