Human/Mouse/Rat p53 HRP-conjugated Antibody

Catalog # Availability Size / Price Qty
HAF1355
Detection of Human p53 by Western Blot.
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Product Details
Citations (11)
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Human/Mouse/Rat p53 HRP-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat p53 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human p53
Asp7-Asp393
Accession # P04637
Formulation
A 0.2 μm filtered solution in Tris with stabilizers and preservatives.
Label
HRP (Horseradish Peroxidase)(Excitation= nm, Emission= nm)

Applications

Recommended Concentration
Sample
Western Blot
1:5000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human p53 antibody by Western Blot. View Larger

Detection of Human p53 by Western Blot. Western blot shows lysates of CEM human T‑lymphoblastoid cell line and MCF‑7 human breast cancer cell line untreated (-) or treated (+) with 10 Gy ionizing radiation (IR) for 1 hour. PVDF membrane was probed with 1:5000 dilution of Human/Mouse/Rat p53 HRP-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # HAF1355). A specific band was detected for p53 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: p53

The p53 tumor suppressor protein is a multi-functional transcription factor that regulates cellular decisions regarding proliferation, cell cycle checkpoints, and apoptosis. The importance of p53 is underscored by its mutation in over 50% of human cancers. Mice that lack one or both copies of p53 also showed an increased incidence of tumors, which makes the p53 deficient mouse a model system for studying cancer generation and progression.

Entrez Gene IDs
7157 (Human); 22059 (Mouse); 24842 (Rat)
Alternate Names
Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53

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Citations for Human/Mouse/Rat p53 HRP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Cross-talk between mutant p53 and p62/SQSTM1 augments cancer cell migration by promoting the degradation of cell adhesion proteins
    Authors: S Mukherjee, M Maddalena, Y Lü, S Martinez, NB Nataraj, A Noronha, S Sinha, K Teng, V Cohen-Kapl, T Ziv, S Arandkar, O Hassin, R Chatterjee, AC Pirona, M Shreberk-S, A Gershoni, Y Aylon, Z Elazar, Y Yarden, D Schramek, M Oren
    Proceedings of the National Academy of Sciences of the United States of America, 2022;119(17):e2119644119.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. ZNF768 links oncogenic RAS to cellular senescence
    Authors: R Villot, A Poirier, I Bakan, K Boulay, E Fernández, R Devillers, L Gama-Braga, L Tribouilla, A Gagné, É Duchesne, D Caron, JS Bérubé, JC Bérubé, Y Coulombe, M Orain, Y Gélinas, S Gobeil, Y Bossé, JY Masson, S Elowe, S Bilodeau, V Manem, P Joubert, FA Mallette, M Laplante
    Nature Communications, 2021;12(1):4841.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Therapeutic vulnerabilities in the DNA damage response for the treatment of ATRX mutant neuroblastoma
    Authors: SL George, F Lorenzi, D King, S Hartlieb, J Campbell, H Pemberton, UH Toprak, K Barker, J Tall, BM da Costa, ML van den Bo, MEM Dolman, JJ Molenaar, HE Bryant, F Westermann, CJ Lord, L Chesler
    EBioMedicine, 2020;59(0):102971.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Angelman syndrome-associated point mutations in the Zn-binding N-terminal (AZUL) domain of UBE3A ubiquitin ligase inhibit binding to the proteasome
    Authors: S Kühnle, G Martinez-N, F Leclere, SD Hayes, JW Harper, PM Howley
    J. Biol. Chem., 2018;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. TRRAP is essential for regulating the accumulation of mutant and wild-type p53 in lymphoma
    Authors: A Jethwa, M S?abicki, J Hüllein, M Jentzsch, V Dalal, S Rabe, L Wagner, T Walther, W Klapper, H Bohnenberg, M Rettel, J Lu, AH Smits, F Stein, MM Savitski, W Huber, Y Aylon, M Oren, T Zenz
    Blood, 2018;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. The MBT repeats of L3MBTL1 link SET8-mediated p53 methylation at lysine 382 to target gene repression.
    Authors: West LE, Roy S, Lachmi-Weiner K
    J. Biol. Chem., 2010;285(48):37725-32.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. KRAB-type zinc-finger protein Apak specifically regulates p53-dependent apoptosis.
    Authors: Tian C, Xing G, Xie P, Lu K, Nie J, Wang J, Li L, Gao M, Zhang L, He F
    Nat. Cell Biol., 2009;11(5):580-91.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Histone methyltransferase protein SETD2 interacts with p53 and selectively regulates its downstream genes.
    Authors: Xie P, Tian C, An L, Nie J, Lu K, Xing G, Zhang L, He F
    Cell. Signal., 2008;20(9):1671-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Arginine methylation of hnRNP K enhances p53 transcriptional activity.
    Authors: Chen Y, Zhou X, Liu N, Wang C, Zhang L, Mo W, Hu G
    FEBS Lett., 2008;582(12):1761-5.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Role for 53BP1 Tudor domain recognition of p53 dimethylated at lysine 382 in DNA damage signaling.
    Authors: Kachirskaia I, Shi X, Yamaguchi H, Tanoue K, Wen H, Wang EW, Appella E, Gozani O
    J. Biol. Chem., 2008;283(50):34660-6.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Western Blot
  11. SVH-B interacts directly with p53 and suppresses the transcriptional activity of p53.
    Authors: Zhou X, Yang G, Huang R, Chen X, Hu G
    FEBS Lett., 2007;581(25):4943-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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