Human/Mouse/Rat p53 HRP-conjugated Antibody

(6 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat p53 in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human p53
    Asp7-Asp393
    Accession # P04637
  • Formulation
    A 0.2 μm filtered solution in Tris with stabilizers and preservatives.
  • Label
    HRP (Horseradish Peroxidase)
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:5000 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human p53 by Western Blot. Western blot shows lysates of CEM human T‑lymphoblastoid cell line and MCF‑7 human breast cancer cell line untreated
(-) or treated (+) with 10 Gy ionizing radiation (IR) for 1 hour. PVDF membrane was probed with 1:5000 dilution of Human/Mouse/Rat p53 HRP-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # HAF1355). A specific band was detected for p53 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Do not freeze.
    • 6 months from date of receipt, 2 to 8 °C as supplied.
Background: p53

The p53 tumor suppressor protein is a multi-functional transcription factor that regulates cellular decisions regarding proliferation, cell cycle checkpoints, and apoptosis. The importance of p53 is underscored by its mutation in over 50% of human cancers. Mice that lack one or both copies of p53 also showed an increased incidence of tumors, which makes the p53 deficient mouse a model system for studying cancer generation and progression.

  • Entrez Gene IDs:
    7157 (Human); 22059 (Mouse); 24842 (Rat)
  • Alternate Names:
    Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Species
Applications
Sample Type
  1. The MBT repeats of L3MBTL1 link SET8-mediated p53 methylation at lysine 382 to target gene repression.
    Authors: West LE, Roy S, Lachmi-Weiner K
    J. Biol. Chem., 2010;285(48):37725-32.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. KRAB-type zinc-finger protein Apak specifically regulates p53-dependent apoptosis.
    Authors: Tian C, Xing G, Xie P, Lu K, Nie J, Wang J, Li L, Gao M, Zhang L, He F
    Nat. Cell Biol., 2009;11(5):580-91.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Role for 53BP1 Tudor domain recognition of p53 dimethylated at lysine 382 in DNA damage signaling.
    Authors: Kachirskaia I, Shi X, Yamaguchi H, Tanoue K, Wen H, Wang EW, Appella E, Gozani O
    J. Biol. Chem., 2008;283(50):34660-6.
    Species: N/A
    Sample Type: Recombinant Protein
    Application: WB
  4. Histone methyltransferase protein SETD2 interacts with p53 and selectively regulates its downstream genes.
    Authors: Xie P, Tian C, An L, Nie J, Lu K, Xing G, Zhang L, He F
    Cell. Signal., 2008;20(9):1671-8.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Arginine methylation of hnRNP K enhances p53 transcriptional activity.
    Authors: Chen Y, Zhou X, Liu N, Wang C, Zhang L, Mo W, Hu G
    FEBS Lett., 2008;582(12):1761-5.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. SVH-B interacts directly with p53 and suppresses the transcriptional activity of p53.
    Authors: Zhou X, Yang G, Huang R, Chen X, Hu G
    FEBS Lett., 2007;581(25):4943-8.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB

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