Human/Mouse/Rat PGK1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human/Mouse/Rat PGK1 by Western Blot.
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Product Details
Citations (1)
Supplemental Products

Human/Mouse/Rat PGK1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects endogenous human, mouse and rat PGK1 in Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PGK1
Accession # P00558
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat PGK1 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat PGK1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, U937 human histiocytic lymphoma cell line, BaF3 mouse pro-B cell line, A20 mouse B cell lymphoma cell line, Rat-2 rat embryonic fibroblast cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PGK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5455) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PGK1 at approximately 43 to 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PGK1

Phosphoglycerate kinase-1 (PGK1) is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3 phosphoglycerate. The gene encoding PGK1 is X-linked. Mutations of this gene may cause phosphoglycerate kinase deficiency, which is characterized by hemolytic anemia, muscle stiffness and mental retardation (1‑3). PGK1 is induced by oxidative stress through the induction of hypoxia-inducible factor 1 alpha and is a potential biomarker and therapeutic target for cancer (4‑7).

  1. Beutler, E. (2002) Br. J. Haematol. 136:3.
  2. Flanagan, J.M. et al. (2006) Br. J. Haematol. 134:233.
  3. Svaasand, E.K. et al. (2007) Muscle Nerve. 36:579.
  4. Hwang, T.L. et al. (2006) Proteomics. 6:2259.
  5. Wang, J. et al. (2007) Cancer Res. 67:149.
  6. Zieker, D. et al. (2008) Cell Physiol. Biochem. 21:429.
  7. Jang, C.H. et al. (2008) Biosci. Biotechnol. Biochem. 72:1799.
Long Name
Phosphoglycerate Kinase-1
Entrez Gene IDs
5230 (Human); 18655 (Mouse); 24644 (Rat)
Alternate Names
Cell migration-inducing gene 10 protein; EC; MGC142128; MGC8947; MIG10; PGK1; PGKA; PGKAMGC117307; phosphoglycerate kinase 1; Primer recognition protein 2; PRP 2; PRP2

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Citation for Human/Mouse/Rat PGK1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Metabolic alterations derived from absence of Two-Pore Channel 1 at cardiac level
    Authors: Francisca Lago
    J. Biosci., 2016;41(4):643-658.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot


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Reconstitution Buffers

Secondary Antibodies

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