Human/Mouse/Rat PGK1 Antibody Summary
Accession # P00558
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human/Mouse/Rat PGK1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, U937 human histiocytic lymphoma cell line, BaF3 mouse pro-B cell line, A20 mouse B cell lymphoma cell line, Rat-2 rat embryonic fibroblast cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PGK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5455) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PGK1 at approximately 43 to 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Phosphoglycerate kinase-1 (PGK1) is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3 phosphoglycerate. The gene encoding PGK1 is X-linked. Mutations of this gene may cause phosphoglycerate kinase deficiency, which is characterized by hemolytic anemia, muscle stiffness and mental retardation (1‑3). PGK1 is induced by oxidative stress through the induction of hypoxia-inducible factor 1 alpha and is a potential biomarker and therapeutic target for cancer (4‑7).
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- Flanagan, J.M. et al. (2006) Br. J. Haematol. 134:233.
- Svaasand, E.K. et al. (2007) Muscle Nerve. 36:579.
- Hwang, T.L. et al. (2006) Proteomics. 6:2259.
- Wang, J. et al. (2007) Cancer Res. 67:149.
- Zieker, D. et al. (2008) Cell Physiol. Biochem. 21:429.
- Jang, C.H. et al. (2008) Biosci. Biotechnol. Biochem. 72:1799.
Citation for Human/Mouse/Rat PGK1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Metabolic alterations derived from absence of Two-Pore Channel 1 at cardiac level
Authors: Francisca Lago
J. Biosci., 2016;41(4):643-658.
Sample Types: Tissue Homogenates
Applications: Western Blot
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