hh Human/Mouse/Rat PGK1 Antibody AF5455: R&D Systems

Human/Mouse/Rat PGK1 Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects endogenous human, mouse and rat PGK1 in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PGK1
    Met1-Ile417
    Accession # P00558
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Mouse/Rat PGK1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, U937 human histiocytic lymphoma cell line, BaF3 mouse pro‑B cell line, A20 mouse B cell lymphoma cell line, Rat‑2 rat embryonic fibroblast cell line, and Y3‑Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PGK1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF5455) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PGK1 at approximately 43 to 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PGK1

Phosphoglycerate kinase-1 (PGK1) is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3 phosphoglycerate. The gene encoding PGK1 is X-linked. Mutations of this gene may cause phosphoglycerate kinase deficiency, which is characterized by hemolytic anemia, muscle stiffness and mental retardation (1‑3). PGK1 is induced by oxidative stress through the induction of hypoxia-inducible factor 1 alpha and is a potential biomarker and therapeutic target for cancer (4‑7).

  • References:
    1. Beutler, E. (2002) Br. J. Haematol. 136:3.
    2. Flanagan, J.M. et al. (2006) Br. J. Haematol. 134:233.
    3. Svaasand, E.K. et al. (2007) Muscle Nerve. 36:579.
    4. Hwang, T.L. et al. (2006) Proteomics. 6:2259.
    5. Wang, J. et al. (2007) Cancer Res. 67:149.
    6. Zieker, D. et al. (2008) Cell Physiol. Biochem. 21:429.
    7. Jang, C.H. et al. (2008) Biosci. Biotechnol. Biochem. 72:1799.
  • Long Name:
    Phosphoglycerate Kinase-1
  • Entrez Gene IDs:
    5230 (Human); 18655 (Mouse); 24644 (Rat)
  • Alternate Names:
    Cell migration-inducing gene 10 protein; EC 2.7.2.3; MGC142128; MGC8947; MIG10; PGK1; PGKA; PGKAMGC117307; phosphoglycerate kinase 1; Primer recognition protein 2; PRP 2; PRP2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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