Human/Mouse/Rat Phospho-Chk1 (S317) Antibody

Catalog # Availability Size / Price Qty
AF2054
AF2054-SP
Detection of Human Phospho-Chk1 (S317) by Western Blot.
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Product Details
Citations (6)
FAQs
Supplemental Products
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Human/Mouse/Rat Phospho-Chk1 (S317) Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Chk1 when phosphorylated at S317 in Western blots. Does not recognize Chk1 when unphosphorylated at S317.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human Chk1 S317 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Phospho-Chk1 (S317) by Western Blot. View Larger

Detection of Human Phospho-Chk1 (S317) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or exposed (+) to 50 J/m2 UV-C for the indicated time. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-Chk1 (S317) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2054) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-Chk1 (S317) was detected at approximately 56 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda -phosphatase ( lambda -PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Phospho-Chk1 (S317) by Simple WesternTM. View Larger

Detection of Human Phospho-Chk1 (S317) by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 50 J/m2 ultraviolet light (UV) for 2 hours, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Chk1 (S317) at approximately 60 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Chk1 (S317) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2054). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chk1

The Chk1 checkpoint kinase is an integral member of a signaling cascade that controls cell cycle progression. In response to genotoxic or replicative stress, Chk1 is phosphorylated by ATM or ATM-related kinases (ATR) at S317. In turn, Chk1 phosphorylates downstream effectors, such as p53 or the Cdc25 phosphatases to halt cell cycle progression and allow time for repair of incurred damage.

Long Name
Checkpoint Kinase 1
Entrez Gene IDs
1111 (Human); 12649 (Mouse); 140583 (Rat)
Alternate Names
Checkpoint, S. pombe, homolog of, 1; CHEK1; CHK1 (checkpoint, S.pombe) homolog; CHK1 checkpoint homolog (S. pombe); Chk1; CHK1serine/threonine-protein kinase Chk1; EC 2.7.11; EC 2.7.11.1

Product Datasheets

Citations for Human/Mouse/Rat Phospho-Chk1 (S317) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Dbf4 is direct downstream target of ataxia telangiectasia mutated (ATM) and ataxia telangiectasia and Rad3-related (ATR) protein to regulate intra-S-phase checkpoint.
    Authors: Lee AY, Chiba T, Truong LN
    J. Biol. Chem., 2012;287(4):2531-43.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  2. Poly(ADP-Ribose) polymerase inhibition synergizes with 5-fluorodeoxyuridine but not 5-fluorouracil in ovarian cancer cells.
    Authors: Huehls AM, Wagner JM, Huntoon CJ, Geng L, Erlichman C, Patel AG, Kaufmann SH, Karnitz LM
    Cancer Res., 2011;71(14):4944-54.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  3. Functional connection between Rad51 and PML in homology-directed repair.
    Authors: Boichuk S, Hu L, Makielski K, Pandolfi PP, Gjoerup OV
    PLoS ONE, 2011;6(10):e25814.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  4. Multiple DNA damage signaling and repair pathways deregulated by simian virus 40 large T antigen.
    Authors: Boichuk S, Hu L, Hein J
    J. Virol., 2010;84(16):8007-20.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  5. Simian virus 40 large T antigen disrupts genome integrity and activates a DNA damage response via Bub1 binding.
    Authors: Hein J, Boichuk S, Wu J, Cheng Y, Freire R, Jat PS, Roberts TM, Gjoerup OV
    J. Virol., 2009;83(1):117-27.
    Species: Human
    Sample Types: Whole Cells
    Applications: WB
  6. RNF8-dependent and RNF8-independent regulation of 53BP1 in response to DNA damage.
    Authors: Sakasai R, Tibbetts R
    J. Biol. Chem., 2008;283(20):13549-55.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB

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