Human/Mouse/Rat Phospho-p70 S6 Kinase (T229) Antibody

Newer Version Available: AF869

Discontinued Product

AF8961 has been discontinued and is replaced by AF869.

Detection of Human Phospho-p70 S6 Kinase (T229) and p85 S6 Kinase (T252) by Western Blot.
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Product Details
Citations (5)

Human/Mouse/Rat Phospho-p70 S6 Kinase (T229) Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human p70 S6 Kinase/p85 S6 Kinase when phosphorylated at T229/T252, respectively. The corresponding sequences of mouse and rat p70 S6K (T229)/p85 S6K (T252) are identical to the phosphopeptide antigen.
Polyclonal Rabbit IgG
Antigen Affinity-purified
Phosphopeptide containing human, mouse, and rat p70 S6 Kinase T229 site
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-p70 S6 Kinase (T229) and p85 S6 Kinase (T252) antibody by Western Blot. View Larger

Detection of Human Phospho-p70 S6 Kinase (T229) and p85 S6 Kinase (T252) by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 15 minutes or 100 ng/mL Recombinant Human IGF-1 (Catalog # 291-G1) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Human Phospho-p70 S6 Kinase (T229) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8961), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-p70 S6 Kinase (T229) and p85 S6 Kinase (T252) at approximately 70 and 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p70 S6 Kinase

p70 S6 kinase is a Ser/Thr kinase activated by such mitogens as EGF, IGF-I, and insulin. The S6 protein of the 40S ribosomal subunit is a major substrate of p70 S6 kinase, and its phosphorylation upregulates the translation of mRNAs containing an oligopyrimidine tract at their transcriptional start site. The activity of p70 S6 kinase is controlled by multiple phosphorylation events, with phosphorylation at T229 by PDK1 and T389 by mTOR most critical for kinase function.

Entrez Gene IDs
6198 (Human); 72508 (Mouse); 83840 (Rat)
Alternate Names
EC 2.7.11; EC; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6 Kinase; p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1; p70 S6 kinase, alpha 2; p70 S6KA; p70 S6K-alpha; p70(S6K)-alpha; p70-alpha; p70-S6K; P70S6K1; PS6K; ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase I; ribosomal protein S6 kinase, 70kD, polypeptide 1; ribosomal protein S6 kinase, 70kDa, polypeptide 1; RPS6KB1; S6K; S6K1; S6K1p70-S6K 1; S6K-beta-1; serine/threonine kinase 14 alpha; Serine/threonine-protein kinase 14A; STK14A

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Citations for Human/Mouse/Rat Phospho-p70 S6 Kinase (T229) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. PDK-1/AKT pathway as a novel therapeutic target in rhabdomyosarcoma cells using OSU-03012 compound.
    Authors: Cen L, Hsieh FC, Lin HJ, Chen CS, Qualman SJ, Lin J
    Br. J. Cancer, 2007-09-17;97(6):785-91.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. ATG1, an autophagy regulator, inhibits cell growth by negatively regulating S6 kinase.
    Authors: Lee SB, Kim S, Lee J, Park J, Lee G, Kim Y, Kim JM, Chung J
    EMBO Rep., 2007-03-09;8(4):360-5.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Overcoming trastuzumab resistance in HER2-overexpressing breast cancer cells by using a novel celecoxib-derived phosphoinositide-dependent kinase-1 inhibitor.
    Authors: Tseng PH, Wang YC, Weng SC, Weng JR, Chen CS, Brueggemeier RW, Shapiro CL, Chen CY, Dunn SE, Pollak M, Chen CS
    Mol. Pharmacol., 2006-08-03;70(5):1534-41.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Troglitazone, a peroxisome proliferator-activated receptor-gamma agonist, decreases tau phosphorylation in CHOtau4R cells.
    Authors: d'Abramo C, Ricciarelli R, Pronzato MA, Davies P
    J. Neurochem., 2006-06-19;98(4):1068-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  5. IGF-I elicits growth of human intestinal smooth muscle cells by activation of PI3K, PDK-1, and p70S6 kinase.
    Authors: Kuemmerle JF
    Am. J. Physiol. Gastrointest. Liver Physiol., 2002-11-20;284(3):G411-22.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot


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