Human/Mouse/Rat Phospho-p70 S6 Kinase (T229) Antibody

Catalog #: AF8961
5 Citations Datasheet / COA / SDS

Newer Version Available: AF869

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Discontinued Product

AF8961 has been discontinued and is replaced by AF869.

Detection of Human Phospho-p70 S6 Kinase (T229) and p85 S6 Kinase (T252) by Western Blot.

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Citations (5)

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Human/Mouse/Rat Phospho-p70 S6 Kinase (T229) Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human p70 S6 Kinase/p85 S6 Kinase when phosphorylated at T229/T252, respectively. The corresponding sequences of mouse and rat p70 S6K (T229)/p85 S6K (T252) are identical to the phosphopeptide antigen.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide containing human, mouse, and rat p70 S6 Kinase T229 site

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-p70 S6 Kinase (T229) and p85 S6 Kinase (T252) by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 15 minutes or 100 ng/mL Recombinant Human IGF-1 (Catalog # 291-G1) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Human Phospho-p70 S6 Kinase (T229) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8961), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-p70 S6 Kinase (T229) and p85 S6 Kinase (T252) at approximately 70 and 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p70 S6 Kinase

p70 S6 kinase is a Ser/Thr kinase activated by such mitogens as EGF, IGF-I, and insulin. The S6 protein of the 40S ribosomal subunit is a major substrate of p70 S6 kinase, and its phosphorylation upregulates the translation of mRNAs containing an oligopyrimidine tract at their transcriptional start site. The activity of p70 S6 kinase is controlled by multiple phosphorylation events, with phosphorylation at T229 by PDK1 and T389 by mTOR most critical for kinase function.

Entrez Gene IDs

6198 (Human); 72508 (Mouse); 83840 (Rat)

Alternate Names

EC 2.7.11; EC 2.7.11.1; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6 Kinase; p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1; p70 S6 kinase, alpha 2; p70 S6KA; p70 S6K-alpha; p70(S6K)-alpha; p70-alpha; p70-S6K; P70S6K1; PS6K; ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase I; ribosomal protein S6 kinase, 70kD, polypeptide 1; ribosomal protein S6 kinase, 70kDa, polypeptide 1; RPS6KB1; S6K; S6K1; S6K1p70-S6K 1; S6K-beta-1; serine/threonine kinase 14 alpha; Serine/threonine-protein kinase 14A; STK14A

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat Phospho-p70 S6 Kinase (T229) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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PDK-1/AKT pathway as a novel therapeutic target in rhabdomyosarcoma cells using OSU-03012 compound.
Authors: Cen L, Hsieh FC, Lin HJ, Chen CS, Qualman SJ, Lin J
Br. J. Cancer, 2007-09-17;97(6):785-91.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
ATG1, an autophagy regulator, inhibits cell growth by negatively regulating S6 kinase.
Authors: Lee SB, Kim S, Lee J, Park J, Lee G, Kim Y, Kim JM, Chung J
EMBO Rep., 2007-03-09;8(4):360-5.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Overcoming trastuzumab resistance in HER2-overexpressing breast cancer cells by using a novel celecoxib-derived phosphoinositide-dependent kinase-1 inhibitor.
Authors: Tseng PH, Wang YC, Weng SC, Weng JR, Chen CS, Brueggemeier RW, Shapiro CL, Chen CY, Dunn SE, Pollak M, Chen CS
Mol. Pharmacol., 2006-08-03;70(5):1534-41.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Troglitazone, a peroxisome proliferator-activated receptor-gamma agonist, decreases tau phosphorylation in CHOtau4R cells.
Authors: d'Abramo C, Ricciarelli R, Pronzato MA, Davies P
J. Neurochem., 2006-06-19;98(4):1068-77.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot
IGF-I elicits growth of human intestinal smooth muscle cells by activation of PI3K, PDK-1, and p70S6 kinase.
Authors: Kuemmerle JF
Am. J. Physiol. Gastrointest. Liver Physiol., 2002-11-20;284(3):G411-22.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot