Human/Mouse/Rat PLC‑ gamma 1 Antibody

Name: Human/Mouse/Rat PLC‑ gamma 1 Antibody
Brand: R&D Systems
SKU: MAB3288
Price: 459 USD
Availability: InStock

R&D Systems | Catalog # MAB3288

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 346130

View all PLC-gamma 1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PLC-gamma 1
Ser1091-Leu1290
Accession # P19174

Specificity

Detects endogenous human, mouse, and rat PLC-gamma 1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human/Mouse/Rat PLC‑ gamma 1 Antibody

Detection of Human/Mouse/Rat PLC-gamma 1 by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line, Neuro-2A mouse neuroblastoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PLC-gamma 1 Monoclonal Antibody (Catalog # MAB3288) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PLC- gamma1 at approximately 155 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat PLC‑ gamma 1 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: HEK293 human embryonic kidney cell line, Neuro-2A mouse neuroblastoma cell line, and NRK rat normal kidney cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: PLC-gamma 1

Phospholipase C gamma-1 (PLC-gamma 1) is activated by receptor tyrosine kinases in response to growth factors and hormones. Activated PLC-gamma 1 catalyzes the hydrolysis of phosphatidylinositol 4,5-biphosphate to produce the second messengers inositol 1,4,5-triphosphate (IP₃) and diacylglycerol (DAG). IP₃mobilizes the release of calcium while DAG activates protein kinase C. PLC-gamma 1 plays an important role in regulating cell proliferation and differentiation.