Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PP1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PP1 alpha Catalytic Subunit
Lys41-Pro298
Accession # P62136

Specificity

Detects endogenous human, mouse and rat PP1 alpha in Western blots. Based on the immunogen’s homology, the antibody is expected to cross‑react with PP1 beta and PP1 gamma.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat PP1 Catalytic Subunit Antibody

Detection of Human/Mouse/Rat PP1 Catalytic Subunit, antibody by Western Blot.

Detection of Human/Mouse/Rat PP1 Catalytic Subunit, by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PP1 Catalytic Subunit, Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3000) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PP1 at approximately 39 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat PP1 Catalytic Subunit Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and C2C12 mouse myoblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PP1

Protein Phosphatase 1, also known as PP1 and PPP1, is an enzyme that removes phosphate groups attached to serine or threonine residues in proteins. The holoenzyme is composed of two subunits, a catalytic subunit (PP1ca) that is highly conserved throughout evolution, and a wide variety of regulatory subunits that target the enzyme to specific subcellular compartments and proteins. The catalytic subunit is ubiquitously expressed in tissues and cell lines.

Long Name

Protein Phosphatase 1

Alternate Names

PP1A;PP-1A;PP1alpha;PPP1A;PPP1CA;Serine/threonine-protein phosphatase;Serine/threonine-protein phosphatase PP1-alpha catalytic subunit

Entrez Gene IDs

5499 (Human); 19045 (Mouse); 24668 (Rat)

Gene Symbol

PPP1CA

UniProt

P62136 (Human)

Additional PP1 Products

Product Documents for Human/Mouse/Rat PP1 Catalytic Subunit Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat PP1 Catalytic Subunit Antibody

For research use only

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