Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 498922
View all PP2C alpha/PPM1A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PP2C alpha /PPM1A
Met1-Gly324
Accession # P35813

Specificity

Detects human, mouse, and rat PP2C alpha /PPM1A in Western blots. No cross-reactivity with recombinant human PP2C beta, ILKAP/PP2C delta, or PP2C gamma is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat PP2C alpha /PPM1A Antibody

Detection of Human, Mouse, and Rat PP2Ca/PPM1A antibody by Western Blot.

Detection of Human, Mouse, and Rat PP2C alpha /PPM1A by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat PP2Ca/ PPM1A Monoclonal Antibody (Catalog # MAB4150) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PP2Ca/PPM1A at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat PP2C alpha /PPM1A Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: MCF‑7 human breast cancer cell line, Neuro‑2A mouse neuroblastoma cell line, and Rat‑2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PP2C alpha/PPM1A

Protein phosphatase 2C alpha, also called PP2C alpha, Protein Phosphatase, Magnesium-dependent type 1A, and PPM1A, dephosphorylates proteins with some preference for phospho-threonine over phospho-serine residues (1). It is insensitive to the PP1 and PP2a inhibitors such as okadaic acid, (2) but has an absolute requirement for either magnesium or manganese ions (3) and can be activated by unsaturated fatty acids such as arachidonic and oleic acids (4). Overexpression of PP2C alpha causes G2/M cell cycle arrest and apoptosis that has been associated with the activation of p53 (5). Dephosphorylation of proteins in the nucleus by PP2C alpha is suspected to play a role in terminating or reducing the sensitivity of the responses to SMADS (6) and stress-activated proteins such as p38 and JNK (7).

References

  1. Donella-Deanna, A. et al. (1991) Biochimica et Biophysica Acta 1094:130.
  2. Bialojan, C. and A. Takai (1998) Biochem. J. 256:283.
  3. Cohen, P. Annu. Rev. Biochem. (1989) 58:453.
  4. Klumpp, S. et al. (1998) FEBS Let. 437:229.
  5. Ofek, P. et al. (2003) J. Biol. Chem. 278:14299.
  6. Lin, X. et al. (2006) Cell 125:915.
  7. Takekawa, M. et al. (1998) EMBO J. 17:4744.

Long Name

Protein Phosphatase 2C alpha

Alternate Names

MMPa-2, MPPa-1, Pp2c1, PPM1A

Entrez Gene IDs

5494 (Human); 19042 (Mouse); 24666 (Rat)

Gene Symbol

PPM1A

UniProt

P35813

Additional PP2C alpha/PPM1A Products

Product Documents for Human/Mouse/Rat PP2C alpha /PPM1A Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat PP2C alpha /PPM1A Antibody

For research use only

Citations for Human/Mouse/Rat PP2C alpha /PPM1A Antibody

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