Detection of Human, Mouse, and Rat PP2C alpha /PPM1A by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, Neuro‑2A mouse neuroblastoma cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat PP2C alpha / PPM1A Monoclonal Antibody (Catalog # MAB4150) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PP2C alpha /PPM1A at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PP2C alpha/PPM1A
Protein phosphatase 2C alpha, also called PP2C alpha, Protein Phosphatase, Magnesium-dependent type 1A, and PPM1A, dephosphorylates proteins with some preference for phospho-threonine over phospho-serine residues (1). It is insensitive to the PP1 and PP2a inhibitors such as okadaic acid, (2) but has an absolute requirement for either magnesium or manganese ions (3) and can be activated by unsaturated fatty acids such as arachidonic and oleic acids (4). Overexpression of PP2C alpha causes G2/M cell cycle arrest and apoptosis that has been associated with the activation of p53 (5). Dephosphorylation of proteins in the nucleus by PP2C alpha is suspected to play a role in terminating or reducing the sensitivity of the responses to SMADS (6) and stress-activated proteins such as p38 and JNK (7).
Donella-Deanna, A. et al. (1991) Biochimica et Biophysica Acta 1094:130.
Bialojan, C. and A. Takai (1998) Biochem. J. 256:283.
Cohen, P. Annu. Rev. Biochem. (1989) 58:453.
Klumpp, S. et al. (1998) FEBS Let. 437:229.
Ofek, P. et al. (2003) J. Biol. Chem. 278:14299.
Lin, X. et al. (2006) Cell 125:915.
Takekawa, M. et al. (1998) EMBO J. 17:4744.
Protein Phosphatase 2C alpha
Entrez Gene IDs:
5494 (Human); 19042 (Mouse); 24666 (Rat)
EC 220.127.116.11; FLJ42306; MGC9201; MMPa-2; MPPa-1; phosphatase 2C alpha; Pp2c1; PP2CA; PP2Calpha; PP2C-alpha; PPM1A; PPPM1A; protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform; protein phosphatase 1A; protein phosphatase 2C alpha isoform; Protein phosphatase 2C isoform alpha; Protein phosphatase IA; protein phosphatase, Mg2+/Mn2+ dependent, 1A
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