Detection of Human, Mouse, and Rat PTP‑MEG2 by Western Blot. Western blot shows lysates of HEL 92.1.7 human erythroleukemic cell line, CTLL‑2 mouse cytotoxic T cell line, and Nb2‑11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat PTP‑MEG2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2668) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PTP‑MEG2 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Megakaryocyte protein tyrosine phosphatase 2 (PTP-MEG2), also known as PTPN9, contains an N-terminal domain homologous to the yeast retinaldehyde binding domain Sec14p and a C-terminal phosphatase domain. The Sec14p domain targets PTP-MEG2 to the membrane of secretory vesicles, where it promotes vesicle fusion. Elevated levels of membrane bound PTP-MEG2 activity in erythroid progenitor cells are observed in polycythemia vera and may affect responsiveness to colony stimulating factors.
Megakaryocyte Protein Tyrosine Phosphatase 2
Entrez Gene IDs:
5780 (Human); 56294 (Mouse); 266611 (Rat)
EC 184.108.40.206; MEG2; protein tyrosine phosphatase, non-receptor type 9; Protein-tyrosine phosphatase MEG2; PTPase MEG2; PTPase-MEG2; PTPMEG2; PTP-MEG2; PTPN9; tyrosine-protein phosphatase non-receptor type 9
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