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Human PTP1B Antibody

Catalog #: AF13661
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF13661
AF13661-SP
Detection of Human PTP1B by Western Blot.
2 Images
Product Details
Citations (2)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PTP1B Antibody Summary

Species Reactivity
Human
Specificity
Detects human PTP1B in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PTP1B
Glu2-Asn321
Accession # P18031
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5-1 µg/mL
TF‑1 human erythroleukemic cell line, CHP‑100 human neuroblastoma cell line, and HeLa human cervical epithelial carcinoma cell line.
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PTP1B antibody by Western Blot. View Larger

Detection of Human PTP1B by Western Blot. Western blot shows lysates of TF-1 human erythroleukemic cell line and CHP-100 human neuroblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF13661) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PTP1B at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human PTP1B antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human PTP1B by Simple WesternTM. Simple Western lane view shows lysates of MCF-7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for PTP1B at approximately 57 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF13661) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTP1B

Protein tyrosine phosphatase 1B (PTP1B) is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This ubiquitously expressed enzyme is anchored in the endoplasmic reticulum by its C-terminal end and has its catalytic regions exposed to the cytosol. The recombinant protein lacks the C-terminal 114 amino acids but is fully active. PTP1B will dephosphorylate a wide variety of phosphoproteins, such as receptors for the growth factors insulin and epidermal growth factor (EGF), c-Src and beta -catenin. Of particular interest is the observation that PTP1B knock-out mice are resistant to high-caloric intake-induced obesity and have exaggerated insulin responses, suggesting that PTP1B may play an important role in regulating growth factor responsiveness.

References
  1. Angers-Loustau, et al. (1999) Biochem. Cell Biol. 77:493.
  2. Sarmiento, et al. (1998) J. Biol. Chem. 273:26368.
  3. Bjorge, et al. (2000) J. Biol. Chem. 52:41439.
  4. Balsamo, et al. (1996) J. Cell Biol. 134:801.
  5. Elchebly, et al. (1999) Science 283:1544.
Long Name
Protein Tyrosine Phosphatase 1B
Entrez Gene IDs
5770 (Human); 19246 (Mouse)
Alternate Names
EC 3.1.3.48; protein tyrosine phosphatase 1B; protein tyrosine phosphatase, non-receptor type 1; Protein-tyrosine phosphatase 1B; PTP1B; PTP-1B; PTP1Bprotein tyrosine phosphatase, placental; PTPN1; tyrosine-protein phosphatase non-receptor type 1

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Citations for Human PTP1B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Maternal high-fat diet alters lung development and function in the offspring
    Authors: Kathryn M. Heyob, Saya Mieth, Sophia S. Sugar, Amanda E. Graf, Scott W. Lallier, Rodney D. Britt et al.
    American Journal of Physiology-Lung Cellular and Molecular Physiology
  2. Molecular network analysis of phosphotyrosine and lipid metabolism in hepatic PTP1b deletion mice
    Authors: Emily R. Miraldi, Hadar Sharfi, Randall H. Friedline, Hannah Johnson, Tejia Zhang, Ken S. Lau et al.
    Integrative Biology

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