Human/Mouse/Rat PTP1B Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse and rat PTP1B in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human PTP1B
    Glu2-Asn321
    Accession # P18031
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Mouse/Rat PTP1B by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, PC-12 rat adrenal pheochromocytoma cell line, and L-929 mouse fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF13661) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PTP1B at approximately 50 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Detection of Human PTP1B by Simple WesternTM. Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for PTP1B at approximately 57 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF13661) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PTP1B

Protein tyrosine phosphatase 1B (PTP1B) is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This ubiquitously expressed enzyme is anchored in the endoplasmic reticulum by its C-terminal end and has its catalytic regions exposed to the cytosol. The recombinant protein lacks the C-terminal 114 amino acids but is fully active. PTP1B will dephosphorylate a wide variety of phosphoproteins, such as receptors for the growth factors insulin and epidermal growth factor (EGF), c-Src and beta -catenin. Of particular interest is the observation that PTP1B knock-out mice are resistant to high-caloric intake-induced obesity and have exaggerated insulin responses, suggesting that PTP1B may play an important role in regulating growth factor responsiveness.

  • References:
    1. Angers-Loustau, et al. (1999) Biochem. Cell Biol. 77:493.
    2. Sarmiento, et al. (1998) J. Biol. Chem. 273:26368.
    3. Bjorge, et al. (2000) J. Biol. Chem. 52:41439.
    4. Balsamo, et al. (1996) J. Cell Biol. 134:801.
    5. Elchebly, et al. (1999) Science 283:1544.
  • Long Name:
    Protein Tyrosine Phosphatase 1B
  • Entrez Gene IDs:
    5770 (Human); 19246 (Mouse)
  • Alternate Names:
    EC 3.1.3.48; protein tyrosine phosphatase 1B; protein tyrosine phosphatase, non-receptor type 1; Protein-tyrosine phosphatase 1B; PTP1B; PTP-1B; PTP1Bprotein tyrosine phosphatase, placental; PTPN1; tyrosine-protein phosphatase non-receptor type 1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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