Human/Mouse/Rat Raf-1 Alexa Fluor® 405-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Raf-1

The Raf serine/threonine kinases are effectors of Ras that function as MAP3Ks in the ERK phosphorylation cascade. Mammals express three Raf proteins: A-Raf, B-Raf, and Raf-1, also known as C-Raf. Human Raf-1 contains three distinct regions; an N-terminal RBD (Ras-binding domain) (aa 56 - 131), followed by two rich segments [a cysteine-finger region (aa 138 - 184) (also called CR1/C1) and a second cysteine-rich region (CR2) (aa 253 - 264)] and a C-terminal Ser/Thr kinase catalytic domain (aa 354 - 611). Active Raf-1 phosphorylates and activates the MAPK kinases MEK1 and 2, which in turn phosphorylate and activate the MAP kinases ERK1 and 2.

Product Datasheets

Related Research Areas

• Chemokine Receptor Intracellular Signaling
• Common gamma Chain Receptor Family
• C-type Lectin Receptors
• Intracellular Kinases
• Intracellular Kinases in the Akt Pathway
• Intracellular Signaling Molecules in Angiogenesis
• PDGF Family
• ROS and Stress Resistance
• Search Kinases and Phosphatases
• TNF Superfamily Regulators
• VEGF Family