Human/Mouse/Rat Raf-1 Antibody

Catalog # Availability Size / Price Qty
MAB4540
MAB4540-SP
Human/Mouse/Rat Raf-1 Antibody in Western Blot
2 Images
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (2)

Human/Mouse/Rat Raf-1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Raf-1 at 74 kDa in Western blots.
Source
Monoclonal Mouse IgG2A Clone # 563002
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Raf-1
Asn189-Thr353
Accession # P04049
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat Raf‑1 by Western Blot. View Larger

Detection of Human, Mouse, and Rat Raf‑1 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, A431 human epithelial carcinoma cell line, L-929 mouse fibroblast cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Raf-1 Monoclonal Antibody (Catalog # MAB4540) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Raf-1 at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunohistochemistry Raf‑1 in Human Liver. View Larger

Raf‑1 in Human Liver. Raf-1 was detected in immersion fixed paraffin-embedded sections of human liver array using Mouse Anti-Human/Mouse/Rat Raf-1 Monoclonal Antibody (Catalog # MAB4540) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Raf-1

The Raf serine/threonine kinases are effectors of Ras that function as MAP3Ks in the ERK phosphorylation cascade. Mammals express three Raf proteins: A-Raf, B-Raf, and Raf-1, also known as C-Raf. Human Raf-1 contains three distinct regions; an N-terminal RBD (Ras-binding domain) (aa 56 - 131), followed by two rich segments [a cysteine-finger region (aa 138 - 184) (also called CR1/C1) and a second cysteine-rich region (CR2) (aa 253 - 264)] and a C-terminal Ser/Thr kinase catalytic domain (aa 354 - 611). Active Raf-1 phosphorylates and activates the MAPK kinases MEK1 and 2, which in turn phosphorylate and activate the MAP kinases ERK1 and 2.

Long Name
v-raf-1Murine Leukemia Viral Oncogene Homolog 1
Entrez Gene IDs
5894 (Human); 110157 (Mouse); 24703 (Rat)
Alternate Names
cRaf; c-Raf; c-Raf-1; CRAFraf proto-oncogene serine/threonine protein kinase; EC 2.7.11; EC 2.7.11.1; NS5; Proto-oncogene c-RAF; RAF proto-oncogene serine/threonine-protein kinase; RAF; Raf1; Raf-1; Raf-1Oncogene RAF1; v-raf-1 murine leukemia viral oncogene homolog 1; v-Raf-1

Product Datasheets

Citation for Human/Mouse/Rat Raf-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Macrocyclic lactones inhibit nasopharyngeal carcinoma cells proliferation through PAK1 inhibition and reduce in vivo tumor growth
    Authors: F Gallardo, B Mariamé, R Gence, AF Tilkin-Mar
    Drug Des Devel Ther, 2018;12(0):2805-2814.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse/Rat Raf-1 Antibody

Average Rating: 4 (Based on 2 Reviews)

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Human/Mouse/Rat Raf-1 Antibody
By Anonymous on 11/18/2019
Application: WB Sample Tested: Hematological cancer cell lines lysates Species: Human

30 ug of protein per sample.
1:500 dilution of primary Ab.


Human/Mouse/Rat Raf-1 Antibody
By Anonymous on 06/06/2019
Application: WB Sample Tested: Lung single-cell suspension Species: Mouse