Human/Mouse/Rat Raf‑1 Alexa Fluor™ Plus 647‑conjugated Antibody

Name: Human/Mouse/Rat Raf‑1 Alexa Fluor™ Plus 647‑conjugated Antibody
Brand: R&D Systems
SKU: FAB4540AFP647
Price: 559 USD
Availability: InStock

R&D Systems | Catalog # FAB4540AFP647

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)

Antibody Source

Monoclonal Mouse IgG_2A Clone # 563002

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Product Specifications

Specificity

Detects human, mouse, and rat Raf-1 at 74 kDa in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Applications for Human/Mouse/Rat Raf‑1 Alexa Fluor™ Plus 647‑conjugated Antibody

Application

Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Raf-1

The Raf serine/threonine kinases are effectors of Ras that function as MAP3Ks in the ERK phosphorylation cascade. Mammals express three Raf proteins: A-Raf, B-Raf, and Raf-1, also known as C-Raf. Human Raf-1 contains three distinct regions; an N-terminal RBD (Ras-binding domain) (aa 56 - 131), followed by two rich segments [a cysteine-finger region (aa 138 - 184) (also called CR1/C1) and a second cysteine-rich region (CR2) (aa 253 - 264)] and a C-terminal Ser/Thr kinase catalytic domain (aa 354 - 611). Active Raf-1 phosphorylates and activates the MAPK kinases MEK1 and 2, which in turn phosphorylate and activate the MAP kinases ERK1 and 2.

Long Name

v-raf-1Murine Leukemia Viral Oncogene Homolog 1

Alternate Names

c-Raf-1, Raf1, v-Raf-1

Entrez Gene IDs

5894 (Human); 110157 (Mouse); 24703 (Rat)

Gene Symbol

RAF1

UniProt

P04049

Additional Raf-1 Products

Product Documents for Human/Mouse/Rat Raf‑1 Alexa Fluor™ Plus 647‑conjugated Antibody

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Product Specific Notices for Human/Mouse/Rat Raf‑1 Alexa Fluor™ Plus 647‑conjugated Antibody

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only