Human/Mouse/Rat RalA Antibody

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Human/Mouse/Rat RalA Antibody in Data
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Product Details
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Human/Mouse/Rat RalA Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human, mouse and rat RalA in Western blots. When immunoblotted, no cross-reactivity with recombinant human (rh) RalB, rhH-Ras, rhN‑Ras or rhK-Ras is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human RalA
Accession # P11233
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human/Mouse/Rat RalA antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat RalA by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MDA-MB-468 human breast cancer cell line, NIH-3T3 mouse embryonic fibroblast cell line, and rat brain tissue. PVDF membrane was probed with 1 µg/mL Human/Mouse/Rat RalA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3270) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human RalA and RalB (1 ng/lane) were included. A specific band for RalA was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RalA

The Ras-like proteins RalA and RalB share 85% identity and constitute a grouping within the Ras superfamily of small GTPases. Like other GTPases, Ral proteins transduce signals by cycling between an active GTP-bound and an inactive GDP-bound state. Ral functions as an effector of Ras-mediated signaling, and has been implicated in the regulation of vesicle trafficking and cell morphology.

Long Name
Ras-like Protein A
Entrez Gene IDs
5898 (Human); 56044 (Mouse); 81757 (Rat)
Alternate Names

Product Datasheets


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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