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Human/Mouse/Rat RalA/RalB Antibody

Catalog #: AF3204
1 Citation Datasheet / COA / SDS
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AF3204
AF3204-SP

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Detection of Human/Mouse/Rat RalA/RalB by Western Blot.
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Citations (1)
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Human/Mouse/Rat RalA/RalB Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat RalA/RalB in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human H-Ras, N-Ras, or K-Ras is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human RalB
Met1-Leu206
Accession # P11234
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
Exosome Standards (HT-29) (Catalog # NBP3-11685) and U937 human histiocytic lymphoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat RalA/RalB antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat RalA/RalB by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat RalA/RalB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3204) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human RalA and RalB (5 ng/lane) were included. A specific band for RalA/RalB was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

N/A View Larger

Detection of Human RalA/RalB by Simple WesternTM. Simple Western shows lysates of Exosome Standards (HT‑29) (NBP3-11685) and U937 human histiocytic lymphoma cell line, loaded at 0.5 mg/ml. A specific band was detected for RalA/RalB at approximately 32 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat RalA/RalB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3204). This experiment was conducted under reducing conditions and using the 2-40kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RalA/RalB

The Ras-like proteins RalA and RalB share 85% identity and constitute a grouping within the Ras superfamily of small GTPases. Like other GTPases, Ral proteins transduce signals by cycling between an active GTP-bound and an inactive GDP-bound state. Ral functions as an effector of Ras-mediated signaling, and has been implicated in the regulation of vesicle trafficking and cell morphology.

Long Name
Ras-like Protein A/B
Alternate Names
MGC48949; RAL; RalA/RalB; Ras family small GTP binding protein RALA; RAS-like protein A; ras-related protein Ral-A; v-ral simian leukemia viral oncogene homolog A (ras related)

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Citation for Human/Mouse/Rat RalA/RalB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Expression of ral GTPases, their effectors, and activators in human bladder cancer.
    Authors: Smith SC, Oxford G, Baras AS, Owens C, Havaleshko D, Brautigan DL, Safo MK, Theodorescu D
    Clin. Cancer Res., 2007-07-01;13(13):3803-13.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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