Human/Mouse/Rat RalA/RalB Antibody Summary
Accession # P11234
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human/Mouse/Rat RalA/RalB by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat RalA/RalB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3204) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human RalA and RalB (5 ng/lane) were included. A specific band for RalA/RalB was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The Ras-like proteins RalA and RalB share 85% identity and constitute a grouping within the Ras superfamily of small GTPases. Like other GTPases, Ral proteins transduce signals by cycling between an active GTP-bound and an inactive GDP-bound state. Ral functions as an effector of Ras-mediated signaling, and has been implicated in the regulation of vesicle trafficking and cell morphology.
Citation for Human/Mouse/Rat RalA/RalB Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Expression of ral GTPases, their effectors, and activators in human bladder cancer.
Authors: Smith SC, Oxford G, Baras AS, Owens C, Havaleshko D, Brautigan DL, Safo MK, Theodorescu D
Clin. Cancer Res., 2007;13(13):3803-13.
Sample Types: Cell Lysates
Applications: Western Blot
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