Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all RKIP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human RKIP
Met1-Lys187
Accession # P30086

Specificity

Detects endogenous human, mouse, and rat RKIP in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat RKIP Antibody

Detection of Human/Mouse/Rat RKIP antibody by Western Blot.

Detection of Human/Mouse/Rat RKIP by Western Blot.

Western blot shows lysates of HUVEC human umbilical vein endothelial cells and A172 human glioblastoma cell line. PVDF membrane was probed with 0.2 µg/mL of Human/Mouse/Rat RKIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4538) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for RKIP at approximately 23 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat RKIP Antibody

Application
Recommended Usage

Western Blot

0.2 µg/mL
Sample: HUVEC human umbilical vein endothelial cells and A172 human glioblastoma cell line

Reviewed Applications

Read 4 reviews rated 3.8 using AF4538 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RKIP

Raf kinase inhibitor protein (RKIP) is a member of the phosphatidylethanolamine-binding protein (PEBP) family, and modulates the activation of several signal transduction cascades. By binding the MAP3K Raf-1, unphosphorylated RKIP inhibits activation of the MAP2K MEK. PKC-phosphorylated RKIP dissociates from Raf‑1 and inhibits GRK2, resulting in sustained G-protein signaling. Expressed in many tissues, RKIP is implicated in the regulation of such physiological processes as membrane biosynthesis, spermatogenesis, neural development, and metastasis suppression.

Long Name

Raf kinase inhibitory protein

Alternate Names

HCNPpp, Neuropolypeptide h3, PBP, PEBP-1, PEBP1

Entrez Gene IDs

5037 (Human); 23980 (Mouse); 29542 (Rat)

Gene Symbol

PEBP1

UniProt

P30086 (Human)

Additional RKIP Products

Product Documents for Human/Mouse/Rat RKIP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat RKIP Antibody

For research use only

Related Research Areas

Serine Proteases and Regulators

Customer Reviews for Human/Mouse/Rat RKIP Antibody (4)

3.8 out of 5
4 Customer Ratings
5 Stars
0%
4 Stars
75%
3 Stars
25%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human/Mouse/Rat RKIP Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    Human/Mouse/Rat RKIP Antibody AF4538
  • Human/Mouse/Rat RKIP Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/14/2018
  • Human/Mouse/Rat RKIP Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Plasma
    Species: Human
    Verified Customer | Posted 11/10/2018
  • Human/Mouse/Rat RKIP Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/02/2018

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