Human/Mouse/Rat ROCK1 Antibody

Catalog # Availability Size / Price Qty
MAB4590
MAB4590-SP
Human/Mouse/Rat ROCK1 Antibody in Data
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Human/Mouse/Rat ROCK1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human ROCK1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human ROCK2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 701118
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human ROCK1
Ala629-Thr829
Accession # Q13464
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human, Mouse, and Rat ROCK1 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat ROCK1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ROCK1 Monoclonal Antibody (Catalog # MAB4590) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ROCK1 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROCK1

ROCK1 is a ubiquitously expressed serine/threonine kinase that is a downstream target of the small GTPase RhoA. ROCK1 is involved in diverse cellular functions, including smooth muscle contraction, actin cytoskeleton organization, cell adhesion and motility, and gene expression (1). ROCK1 contributes to the development of cardiac fibrosis and induction of fibrogenic cytokines in cardiomyocytes in response to pathological stimuli. ROCK1 knockout mice exhibit reduced perivascular and interstitial fibrosis, which is associated with reduced expression of a variety of extracellular matrix (ECM) proteins and fibrogenic cytokines (2).

References
  1. Zhao, Y.M. et al. (2004) Dev. Biol. 275:183.
  2. Zhang, C. et al. (2006) FASEB J. 20:916.
Long Name
Rho-associated, Coiled-Coil Containing Protein Kinase 1
Entrez Gene IDs
6093 (Human); 19877 (Mouse); 81762 (Rat)
Alternate Names
EC 2.7.11; EC 2.7.11.1; MGC131603; MGC43611; p160 ROCK-1; p160ROCK; p160-ROCK; PRO0435; Renal carcinoma antigen NY-REN-35; Rho kinase; rho-associated protein kinase 1; Rho-associated, coiled-coil containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase 1; ROCK1; ROK beta

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Human/Mouse/Rat ROCK1 Antibody
By Anonymous on 02/25/2019
Application: WB Sample Tested: Spinal cord Species: Mouse