Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 701118
View all ROCK1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ROCK1
Ala629-Thr829
Accession # Q13464

Specificity

Detects human ROCK1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human ROCK2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat ROCK1 Antibody

Detection of Human, Mouse, and Rat ROCK1 antibody by Western Blot.

Detection of Human, Mouse, and Rat ROCK1 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ROCK1 Monoclonal Antibody (Catalog # MAB4590) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ROCK1 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat ROCK1 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB4590 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ROCK1

ROCK1 is a ubiquitously expressed serine/threonine kinase that is a downstream target of the small GTPase RhoA. ROCK1 is involved in diverse cellular functions, including smooth muscle contraction, actin cytoskeleton organization, cell adhesion and motility, and gene expression (1). ROCK1 contributes to the development of cardiac fibrosis and induction of fibrogenic cytokines in cardiomyocytes in response to pathological stimuli. ROCK1 knockout mice exhibit reduced perivascular and interstitial fibrosis, which is associated with reduced expression of a variety of extracellular matrix (ECM) proteins and fibrogenic cytokines (2).

References

  1. Zhao, Y.M. et al. (2004) Dev. Biol. 275:183.
  2. Zhang, C. et al. (2006) FASEB J. 20:916.

Long Name

Rho-associated, Coiled-Coil Containing Protein Kinase 1

Alternate Names

p160ROCK, ROK beta

Entrez Gene IDs

6093 (Human); 19877 (Mouse); 81762 (Rat)

Gene Symbol

ROCK1

UniProt

Q13464

Additional ROCK1 Products

Product Documents for Human/Mouse/Rat ROCK1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat ROCK1 Antibody

For research use only

Related Research Areas

Apoptosis Intracellular Kinases

Citations for Human/Mouse/Rat ROCK1 Antibody

Customer Reviews for Human/Mouse/Rat ROCK1 Antibody (1)

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  • Human/Mouse/Rat ROCK1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Spinal cord
    Species: Mouse
    Verified Customer | Posted 02/25/2019
    Human/Mouse/Rat ROCK1 Antibody MAB4590

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