Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Western Blot, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Secretagogin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Secretagogin
Asn164-Pro276
Accession # O76038

Specificity

Detects mouse Secretagogin in Western blots. Reactivity with human, rat, and other species is expected due to the high sequence homology of the immunogen used.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Secretagogin Antibody

Detection of Human/Mouse/Rat Secretagogin antibody by Western Blot.

Detection of Human/Mouse/Rat Secretagogin by Western Blot.

Western blot shows lysates of beta TC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Secretagogin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4878) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Secretagogin at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human and Mouse Secretagogin antibody by Simple WesternTM.

Detection of Human and Mouse Secretagogin by Simple WesternTM.

Simple Western lane view shows lysates of beta TC-6 mouse beta cell insulinoma cell line and human pancreas tissue, loaded at 0.2 mg/mL. A specific band was detected for Secretagogin at approximately 38 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Secretagogin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4878) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse/Rat Secretagogin Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: beta TC‑6 mouse beta cell insulinoma cell line and human pancreas tissue

Western Blot

1 µg/mL
Sample: beta TC‑6 mouse beta cell insulinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Secretagogin

Secretagogin (SCGN) is a 32 kDa member of the EF-hand family of Ca-binding proteins. It is expressed in select neurons, as well as neuroendocrine cells and pancreatic islets. Human Secretagogin is 276 amino acids (aa) in length and contains six 35 aa EF-hand domains (aa 12‑276). There is one alternative splice variant that shows a 22 aa substitution for aa 28‑276, and which demonstrates no Ca-binding ability. Secretagogin is known to circulate, presumably released during cell apoptosis. Over aa 164‑276, human Secretagogin is more than 97% aa identical to mouse, porcine and canine Secretagogin.

Alternate Names

CALBL, SCGN, SECRET, SEGN, Setagin

Entrez Gene IDs

10590 (Human); 214189 (Mouse); 306942 (Rat)

Gene Symbol

SCGN

UniProt

O76038 (Human)

Additional Secretagogin Products

Product Documents for Human/Mouse/Rat Secretagogin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Secretagogin Antibody

For research use only

Citations for Human/Mouse/Rat Secretagogin Antibody

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