Intracellular Staining by Flow Cytometry
|Detection of STAT3 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human/Mouse/Rat STAT3 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC1799F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). View our protocol for Staining Intracellular Molecules.|
Signal Transducer and Activator of Transcription (STAT) proteins are transcription factors activated in response to cytokine, growth factor, or hormone receptor signaling. STAT3 is an 89-92 kDa, and most widely expressed, member of the STAT-family of molecules. STAT3 is considered a direct transcription factor; that is it does not act in an indirect way such as epigenetically through the regulation of DNA methylation. Activation/phosphorylation of STAT3 results in homodimerization or heterodimerization with STAT1, whereupon it enters the nucleus to principally promote cell cycle progression. The propensity of STAT3 and STAT5 toward cell division is of particular interest in oncology. Over amino acids 1-175 of STAT3 that include a protein-protein interaction domain and an NLS, human, rat and mouse show 100% amino acid sequence identity.