Human/Mouse/Rat STAT3 Fluorescein-conjugated Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    The unconjugated antibody (Catalog # MAB1799) detects human, mouse, and rat STAT3 in Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 232209
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human STAT3
    Met1-Asn175
    Accession # P40763
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of STAT3 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human/Mouse/Rat STAT3 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC1799F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: STAT3

Signal Transducer and Activator of Transcription (STAT) proteins are transcription factors activated in response to cytokine, growth factor, or hormone receptor signaling. STAT3 is an 89-92 kDa, and most widely expressed, member of the STAT-family of molecules. STAT3 is considered a direct transcription factor; that is it does not act in an indirect way such as epigenetically through the regulation of DNA methylation. Activation/phosphorylation of STAT3 results in homodimerization or heterodimerization with STAT1, whereupon it enters the nucleus to principally promote cell cycle progression. The propensity of STAT3 and STAT5 toward cell division is of particular interest in oncology. Over amino acids 1-175 of STAT3 that include a protein-protein interaction domain and an NLS, human, rat and mouse show 100% amino acid sequence identity.

  • Long Name:
    Signal Transducer and Activator of Transcription 3
  • Entrez Gene IDs:
    6774 (Human); 20848 (Mouse); 25125 (Rat)
  • Alternate Names:
    Acute-phase response factor; APRFMGC16063; DNA-binding protein APRF; FLJ20882; HIES; signal transducer and activator of transcription 3 (acute-phase responsefactor); signal transducer and activator of transcription 3; STAT3
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Fluorescein‑conjugated Control Antibody

Ctrl IC0041F 6  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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