Human/Mouse/Rat STAT3 PE‑conjugated Antibody

Name: Human/Mouse/Rat STAT3 PE‑conjugated Antibody
Brand: R&D Systems
SKU: IC1799P
Price: 399 USD
Availability: InStock

R&D Systems | Catalog # IC1799P

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Citations (2)

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Product Specifications
Scientific Data
Applications
Spectra Viewer
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Knockout Validated, Intracellular Staining by Flow Cytometry

Cited:

Immunocytochemistry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG_2B Clone # 232209

View all STAT3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human STAT3
Met1-Asn175
Accession # P40763

Specificity

The unconjugated antibody (Catalog # MAB1799) detects human, mouse, and rat STAT3 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human/Mouse/Rat STAT3 PE‑conjugated Antibody

Detection of STAT3 in HeLa Human Cell Line by Flow Cytometry.

HeLa human cell line was stained with Mouse Anti-Human/Mouse/Rat STAT3 PE-conjugated Monoclonal Antibody (Catalog # IC1799P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

STAT3 Specificity is Shown by Flow Cytometry in Knockout Cell Line.

STAT3 knockout HeLa human cervical epithelial cell line was stained with PE-conjugated Mouse Anti-Human/Mouse STAT3 Monoclonal Antibody (Catalog # IC1799P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). No staining in the STAT3 knockout HeLa cell line was observed. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse/Rat STAT3 PE‑conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: HeLa or Jurkat human acute T cell leukemia cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Knockout Validated

STAT3 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in STAT3 knockout HeLa cell line.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: STAT3

Signal Transducer and Activator of Transcription (STAT) proteins are transcription factors activated in response to cytokine, growth factor, or hormone receptor signaling. STAT3 is an 89-92 kDa, and most widely expressed, member of the STAT-family of molecules. STAT3 is considered a direct transcription factor; that is it does not act in an indirect way such as epigenetically through the regulation of DNA methylation. Activation/phosphorylation of STAT3 results in homodimerization or heterodimerization with STAT1, whereupon it enters the nucleus to principally promote cell cycle progression. The propensity of STAT3 and STAT5 toward cell division is of particular interest in oncology. Over amino acids 1-175 of STAT3 that include a protein-protein interaction domain and an NLS, human, rat and mouse show 100% amino acid sequence identity.

Long Name

Signal Transducer and Activator of Transcription 3

Alternate Names

Acute-phase response factor, APRFMGC16063, DNA-binding protein APRF, FLJ20882, HIES, signal transducer and activator of transcription 3, signal transducer and activator of transcription 3 (acute-phase responsefactor)