Human/Mouse/Rat/Yeast Rad23 Antibody

Catalog # Availability Size / Price Qty
Detection of Human/Mouse/Rat/ Yeast Rad23 by Western Blot.
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Product Details
Supplemental Products

Human/Mouse/Rat/Yeast Rad23 Antibody Summary

Species Reactivity
Human, Mouse, Rat, Yeast
Detects human, mouse, rat, and yeast Rad23 protein in Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant yeast Rad23
Accession # P32628
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat/ Yeast Rad23 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat/ Yeast Rad23 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Raji human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, Nb2-11 rat lymphoma cell line, and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat/Yeast Rad23 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4555) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Rad23 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rad23

Rad23 is an evolutionarily conserved protein that is important for nucleotide excision repair. All eukaryotic Rad23 proteins contain an amino-terminal UbL domain that bears a striking resemblance to ubiquitin. Rad23 has a proteolytic function in DNA repair via its interaction with the 26S proteosome through the UbL domain. The elimination of this domain causes sensitivity to ultraviolet radiation.

Entrez Gene IDs
5886 (Human)
Alternate Names
hHR23; hHR23A; HR23A; Rad23

Product Datasheets

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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