Human/Mouse SHIP Antibody

Catalog # Availability Size / Price Qty
AF10117-100
AF10117-SP
Detection  of Human and Mouse SHIP by Western Blot.
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Human/Mouse SHIP Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects mouse SHIP in direct ELISAs.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse SHIP
Glu874-Thr941
Accession # Q9ES52
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection  of Human and Mouse SHIP by Western Blot. View Larger

Detection of Human and Mouse SHIP by Western Blot. Western blot shows lysates of BaF3 mouse pro-B cell line and RL human non-Hodgkin's lymphoma B cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse SHIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10117) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SHIP at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SHIP

SH2-containing inositol phosphatase (SHIP), also known as INPP5D, is a negative regulator of signal transduction in hematopoietic cells. Targeted disruption of SHIP in mice leads to a myeloproliferative disorder. Several laboratories have demonstrated the presence of multiple forms of SHIP, including 145 kDa, 135 kDa, and
C‑terminal truncated forms at 125 kDa and 110 kDa in some cell types.

Long Name
SH2-containing Inositol Phosphatase
Entrez Gene IDs
3635 (Human); 16331 (Mouse); 54259 (Rat)
Alternate Names
145kD; EC 3.1.3; hp51CNMGC142142; Inositol polyphosphate-5-phosphatase of 145 kDa; inositol polyphosphate-5-phosphatase, 145kDa; INPP5D; MGC104855; SHIP; SHIPMGC142140

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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