Human/Mouse Syntaxin 12 Antibody

Catalog # Availability Size / Price Qty
AF6617-100
AF6617-SP
Syntaxin 12  in Human Brain.
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Human/Mouse Syntaxin 12 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Syntaxin 12 in direct ELISAs. Detects human and mouse Syntaxin 12 in immunohistochemistry.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E.coli-derived recombinant human Syntaxin 12
Met1-Gln200
Accession # Q86Y82
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
1-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry Syntaxin 12  in Human Brain. View Larger

Syntaxin 12 in Human Brain. Syntaxin 12 was detected in immersion fixed paraffin-embedded sections of human brain using Goat Anti-Human/Mouse Syntaxin 12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6617) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry Syntaxin 12  in Mouse Brain. View Larger

Syntaxin 12 in Mouse Brain. Syntaxin 12 was detected in perfusion fixed frozen sections of mouse brain (hippocampus) using Goat Anti-Human/Mouse Syntaxin 12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6617) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in neurons and synaptic vesicles. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Syntaxin 12

Syntaxin 12/STX12 is a 39 kDa member of the Syntaxin family. It is a SNARE that acts to regulate protein transport between late endosomes and the trans-Golgi network. Human STX12 is 276 amino acids (aa) in length, and is a type IV single-pass transmembrane protein. The cytoplasmic region extends from aa 1-248. A coiled-coil region extends from aa 33-131 within that region. From aa 178-240, there is a t-SNARE coiled-coil homology domain. The transmembrane region is located at 249-269, and a vesicular area is located at aa 270-276. Human STX12 shares 92% aa sequence identity with mouse STX12.

Entrez Gene IDs
23673 (Human)
Alternate Names
MGC51957; STX12; STX13; STX14; Syntaxin 12; syntaxin-12

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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