Human/Mouse TRANCE/TNFSF11/RANK L Antibody Summary
Accession # AAC51762
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human TRANCE/TNFSF11/RANK L by Western Blot. Western blot shows lysates of human primary differentiated CD4+Th2 cells. PVDF Membrane was probed with 2 µg/mL of Human TRANCE/TNFSF11/RANK L Monoclonal Antibody (Catalog # MAB6263) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TRANCE/TNFSF11/RANK L at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRANCE/TNFSF11/RANK L
TNF-related activation-induced cytokine (TRANCE; also RANKL, OPGL, and ODF) is a 35 kDa (predicted) type II transmembrane glycoprotein and member of the TNF cytokine family. Human TRANCE is 317 amino acids (aa) in length and contains a 47 aa cytoplasmic region, a 21 aa transmembrane region, and a 249 extracellular domain (ECD), which contains two potential sites of N-linked glycosylation. Splicing variants produce three isoforms for human TRANCE. Isoform 1 is the standard form. In isoform 2, aa corresponding to 1-73 in isoform 1 are missing. In isoform 3, aa 1-47 are missing. Human TRANCE ECD is 83% identical to mouse TRANCE ECD. TRANCE is expressed highest in the peripheral lymph nodes and weaker in the spleen, peripheral blood leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach, and thyroid. TRANCE plays a role in osteoclast differentiation and activation.
Citation for Human/Mouse TRANCE/TNFSF11/RANK L Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Locally delivered ethyl-2,5-dihydroxybenzoate using 3D printed bone implant for promotion of bone regeneration in a osteoporotic animal model
Authors: BJ Kwon, GM Seon, MH Lee, MA Koo, MS Kim, D Kim, JJ Han, D Kim, J Kim, JC Park
Eur Cell Mater, 2018;35(0):1-12.
Sample Types: Cell Lysates
Applications: Western Blot
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