Human/Mouse TRANCE/TNFSF11/RANK L Antibody

Catalog # Availability Size / Price Qty
Detection of Human TRANCE/TNFSF11/RANK L by Western Blot.
1 Image
Product Details
Citations (1)
Supplemental Products

Human/Mouse TRANCE/TNFSF11/RANK L Antibody Summary

Species Reactivity
Human, Mouse
Detects human TRANCE/TNFSF11/RANK L in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse (rm) TRANCE and approximately 25% cross-reactivity with recombinant human (rh) LIGHT is observed. No cross-reactivity with rh4-1BB Ligand, rhAPRIL, rhBAFF, rhEDA, rhEDA-A2, rhFas Ligand, rhGITR Ligand, rhOX40 LIgand, rhTNF-alpha, rhTRAIL, rhTWEAK, or rhVEGI is observed.
Monoclonal Mouse IgG3 Clone # 685809
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human TRANCE/TNFSF11/RANK L
Accession # AAC51762
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TRANCE/TNFSF11/RANK L antibody by Western Blot. View Larger

Detection of Human TRANCE/TNFSF11/RANK L by Western Blot. Western blot shows lysates of human primary differentiated CD4+Th2 cells. PVDF Membrane was probed with 2 µg/mL of Human TRANCE/TNFSF11/RANK L Monoclonal Antibody (Catalog # MAB6263) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TRANCE/TNFSF11/RANK L at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.


TNF-related activation-induced cytokine (TRANCE; also RANKL, OPGL, and ODF) is a 35 kDa (predicted) type II transmembrane glycoprotein and member of the TNF cytokine family.  Human TRANCE is 317 amino acids (aa) in length and contains a 47 aa cytoplasmic region, a 21 aa transmembrane region, and a 249 extracellular domain (ECD), which contains two potential sites of N-linked glycosylation.  Splicing variants produce three isoforms for human TRANCE.  Isoform 1 is the standard form.  In isoform 2, aa corresponding to 1-73 in isoform 1 are missing.  In isoform 3, aa 1-47 are missing.  Human TRANCE ECD is 83% identical to mouse TRANCE ECD.  TRANCE is expressed highest in the peripheral lymph nodes and weaker in the spleen, peripheral blood leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach, and thyroid.  TRANCE plays a role in osteoclast differentiation and activation. 

Long Name
TNF-related Activation-induced Cytokine
Entrez Gene IDs
8600 (Human); 21943 (Mouse); 117516 (Rat); 102137946 (Cynomolgus Monkey)
Alternate Names
CD254 antigen; CD254; ODF; OPGL; OPGLOPTB2; Osteoclast differentiation factor; Osteoprotegerin ligand; RANK L; RANKL; RANKLreceptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; sOdf; TNF-related activation-induced cytokine; TNFSF11; TRANCE; TRANCEODFhRANKL2; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11

Product Datasheets

You must select a language.


Citation for Human/Mouse TRANCE/TNFSF11/RANK L Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Locally delivered ethyl-2,5-dihydroxybenzoate using 3D printed bone implant for promotion of bone regeneration in a osteoporotic animal model
    Authors: BJ Kwon, GM Seon, MH Lee, MA Koo, MS Kim, D Kim, JJ Han, D Kim, J Kim, JC Park
    Eur Cell Mater, 2018;35(0):1-12.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse TRANCE/TNFSF11/RANK L Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse TRANCE/TNFSF11/RANK L Antibody and earn rewards!

Have you used Human/Mouse TRANCE/TNFSF11/RANK L Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review