Human/Mouse Transferrin Antibody

Catalog # Availability Size / Price Qty
AF3987
AF3987-SP
Human/Mouse Transferrin Antibody in Western Blot
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Human/Mouse Transferrin Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects mouse Transferrin in direct ELISAs. Detects human Transferrin in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse plasma-derived Transferrin
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Transferrin by Western Blot. View Larger

Detection of Human Transferrin by Western Blot. Western blot shows purified holo-transferrin, purified apo-transferrin, and human plasma. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Transferrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3987) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Transferrin at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Transferrin in Mouse Thymus. View Larger

Transferrin in Mouse Thymus. Transferrin was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Transferrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3987) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Transferrin

Transferrin (also serotransferrin and siderophilin) is a secreted, monomeric 78-82 kDa glycoprotein member of the transferrin family of molecules. It is synthesized by hepatocytes and serves as a transport vehicle for ferric iron, as well as cobalt and manganese ions. When bound to iron, transferrin is referred to as holo-transferrin (Greek: holo - meaning whole or together). When it is absent iron, it is called apotransferrin (Greek: apo - meaning away or apart). Apotransferrin encounters and binds two ferric iron atoms at the basolateral surface of duodenal epithelium. Here, as holo-transferrin, it circulates and distributes iron to virtually all tissues by binding to transferrin receptor 1. Once bound, holo-transferrin is internalized, iron is released, and the resulting apotransferrin is recylced. Mature mouse apotransferrin is 678 amino acids (aa) in length. It is bilobar in shape, with 330 aa N- and C-terminal lobes that each bind one ferric atom. In the absence of iron, each lobe is "open"; when iron is present, the lobes close, forming a compact structure. Mature mouse apotransferrin is 72% and 88% aa identical to human and rat apotransferrin, respectively.

Entrez Gene IDs
7018 (Human)
Alternate Names
Beta-1 metal-binding globulin; DKFZp781D0156; EC 3.4.21; PRO1557; PRO2086; Serotransferrin; Siderophilin; TF; Transferrin

Product Datasheets

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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