Human/Mouse TRAP220 Antibody Summary
Accession # Q15648
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human TRAP220 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse TRAP220 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5520) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TRAP220 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
TRAP220 (Thyroid hormone receptor associated protein 220 kDa) is a widely expressed member of the Mediator complex subunit 1 family of proteins. It constitutes one of some 25 subunits that make up the Mediator complex. Here, it serves as a scaffold protein that links nuclear hormone receptors to RNA polymerase II. Human TRAP220 is 1581 amino acids (aa) in length. It contains a Mediator complex interaction region (aa 108‑390) and two nuclear hormone receptor binding boxes (aa 600‑670). Phosphorylation of Thr1032 and Thr1457 materially increases TRAP220 activity. There are at least two splice variants. One shows a 22 aa substitution for aa 756‑1581 while another shows a nine aa substitution for aa 548‑1581. Over aa 878‑1031, human TRAP220 shares 92% aa identity with mouse TRAP220.
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