TRAP220 (Thyroid hormone receptor associated protein 220 kDa) is a widely expressed member of the Mediator complex subunit 1 family of proteins. It constitutes one of some 25 subunits that make up the Mediator complex. Here, it serves as a scaffold protein that links nuclear hormone receptors to RNA polymerase II. Human TRAP220 is 1581 amino acids (aa) in length. It contains a Mediator complex interaction region (aa 108‑390) and two nuclear hormone receptor binding boxes (aa 600‑670). Phosphorylation of Thr1032 and Thr1457 materially increases TRAP220 activity. There are at least two splice variants. One shows a 22 aa substitution for aa 756‑1581 while another shows a nine aa substitution for aa 548‑1581. Over aa 878‑1031, human TRAP220 shares 92% aa identity with mouse TRAP220.
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Phe878-Phe1031
Accession # Q15648
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human/Mouse TRAP220 Antibody
Detection of Human TRAP220 by Western Blot.
Western blot shows lysates of 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse TRAP220 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5520) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TRAP220 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.
Applications for Human/Mouse TRAP220 Antibody
Western Blot
Sample: 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line
Reviewed Applications
Read 2 reviews rated 4 using AF5520 in the following applications:
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: TRAP220
Long Name
Alternate Names
Gene Symbol
UniProt
Additional TRAP220 Products
Product Documents for Human/Mouse TRAP220 Antibody
Certificate of Analysis
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Product Specific Notices for Human/Mouse TRAP220 Antibody
For research use only
Related Research Areas
Citations for Human/Mouse TRAP220 Antibody
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Application: Western BlotSample Tested: Liver cellsSpecies: HumanVerified Customer | Posted 04/06/2022
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Application: Immunohistochemistry-ParaffinSample Tested: See PMID 22570438Species: MouseVerified Customer | Posted 01/08/2015
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Protocols
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