Human/Mouse Wnt-4 Alexa Fluor® 405-conjugated Antibody

Catalog #: FAB475V Datasheet / COA / SDS

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FAB475V-100UG

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Human/Mouse Wnt-4 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects mouse Wnt-4 in direct ELISAs and Western blots. In sandwich immunoassays, this antibody is specific for human Wnt-4 as a capture antibody when paired with Sheep Anti-Human Wnt‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog #

Source

Monoclonal Rat IgG2a Clone # 55010

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant mouse Wnt-4
aa 37-76 and 222-295
Accession # P22724

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

ELISA

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Wnt-4

Wnt-4 is a secreted glycoprotein that binds to receptors of the Frizzled family. Wnt-4 is important for multiple embryonic development processes including kidney morphogenesis.

Long Name

Wingless-type MMTV Integration Site Family, Member 4

Entrez Gene IDs

54361 (Human); 22417 (Mouse)

Alternate Names

protein Wnt-4; SERKAL; wingless-type MMTV integration site family, member 4; Wnt4; Wnt-4