ZIC1 Antibody

R&D Systems | Catalog # AF4978

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ZIC1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ZIC1
Met1-Gly198
Accession # Q15915

Specificity

Detects human and mouse ZIC1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ZIC1 Antibody

Detection of Human/Mouse ZIC1 antibody by Western Blot.

Detection of Human/Mouse ZIC1 by Western Blot.

Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse ZIC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4978) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ZIC1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
ZIC1 antibody in Mouse Brain by Immunohistochemistry (IHC-Fr).

ZIC1 in Mouse Brain.

ZIC1 was detected in immersion fixed frozen sections of mouse brain (cerebellum) using 15 µg/mL Goat Anti-Human/Mouse ZIC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4978) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
ZIC1 antibody in Human iPSK3 cells by Immunocytochemistry (ICC).

ZIC1 in Human iPSK3 cells.

ZIC1 was detected in immersion fixed human plasmid-derived induced pluripotent stem cells (iPSK3) using Goat Anti-Human/Mouse ZIC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4978) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Detection of ZIC1 by Immunohistochemistry

Detection of ZIC1 by Immunohistochemistry

Immunostaining for iris epithelial markers.(A) Dot plot (as described in Figure 2A) showing transcript abundances across a subset of iris cell types for the markers shown in (B–D). (B) Flat mounts of untreated iris immunostained as indicated. (C,D) Cross sections of untreated iris immunostained as indicated. OTX refers to an antibody that binds to both OTX1 and OTX2. Scale bars, 100 µm. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/73477), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of ZIC1 by Immunohistochemistry

Detection of ZIC1 by Immunohistochemistry

Immunostaining for iris epithelial markers.(A) Dot plot (as described in Figure 2A) showing transcript abundances across a subset of iris cell types for the markers shown in (B–D). (B) Flat mounts of untreated iris immunostained as indicated. (C,D) Cross sections of untreated iris immunostained as indicated. OTX refers to an antibody that binds to both OTX1 and OTX2. Scale bars, 100 µm. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/73477), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for ZIC1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human plasmid-derived induced pluripotent stem cells (iPSK3)

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse brain (cerebellum)

Western Blot

1 µg/mL
Sample: Human brain tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ZIC1

ZIC1 (zinc-finger protein of the cerebellum 1) is a 54-60 kDa member of the GLI C2H2-type Zn-finger protein family. It is expressed in embryonic sclerotome and dermomyotome and in postmitotic cerebellar granule cells and retinal ganglion cells. ZIC1 is a transcription factor that promotes ApoE expression and blocks activation of Math1, allowing for neuroblast multiplication at the expense of differentiation. Human ZIC1 is 447 amino acids (aa) in length. It contains N-terminal poly Ala and His segments, followed by five C2H2-type Zn-fingers. Although the first two are atypical (aa 225-296), the last three Zn-fingers (aa 302-384) are canonical and bind to GLI1, 2 and 3. There is one potential alternate start site at Met353. Over aa 1-198, human ZIC1 is 99% aa identical to mouse ZIC1.

Long Name

Zic Family Member 1

Alternate Names

ZNF201

Entrez Gene IDs

7545 (Human); 22771 (Mouse); 64618 (Rat)

Gene Symbol

ZIC1

UniProt

Q15915

Additional ZIC1 Products

Product Documents for ZIC1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for ZIC1 Antibody

For research use only

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Protocols

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