Human/Mouse ZIC1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human/Mouse ZIC1 by Western Blot.
3 Images
Product Details
Citations (1)
Supplemental Products

Human/Mouse ZIC1 Antibody Summary

Species Reactivity
Human, Mouse
Detects human and mouse ZIC1 in direct ELISAs and Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human ZIC1
Accession # Q15915
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse ZIC1 antibody by Western Blot. View Larger

Detection of Human/Mouse ZIC1 by Western Blot. Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse ZIC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4978) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ZIC1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry ZIC1 antibody in Mouse Brain by Immunohistochemistry (IHC-Fr). View Larger

ZIC1 in Mouse Brain. ZIC1 was detected in immersion fixed frozen sections of mouse brain (cerebellum) using 15 µg/mL Goat Anti-Human/Mouse ZIC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4978) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunocytochemistry ZIC1 antibody in Human iPSK3 cells by Immunocytochemistry (ICC). View Larger

ZIC1 in Human iPSK3 cells. ZIC1 was detected in immersion fixed human plasmid-derived induced pluripotent stem cells (iPSK3) using Goat Anti-Human/Mouse ZIC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4978) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZIC1

ZIC1 (zinc-finger protein of the cerebellum 1) is a 54-60 kDa member of the GLI C2H2-type Zn-finger protein family. It is expressed in embryonic sclerotome and dermomyotome and in postmitotic cerebellar granule cells and retinal ganglion cells. ZIC1 is a transcription factor that promotes ApoE expression and blocks activation of Math1, allowing for neuroblast multiplication at the expense of differentiation. Human ZIC1 is 447 amino acids (aa) in length. It contains N-terminal poly Ala and His segments, followed by five C2H2-type Zn-fingers. Although the first two are atypical (aa 225-296), the last three Zn-fingers (aa 302-384) are canonical and bind to GLI1, 2 and 3. There is one potential alternate start site at Met353. Over aa 1-198, human ZIC1 is 99% aa identical to mouse ZIC1.

Long Name
Zic Family Member 1
Entrez Gene IDs
7545 (Human); 22771 (Mouse); 64618 (Rat)
Alternate Names
Zic family member 1 (odd-paired homolog, Drosophila); ZIC1; ZICZic family member 1 (odd-paired Drosophila homolog); Zinc finger protein 201; Zinc finger protein of the cerebellum 1; ZNF201; ZNF201zinc finger protein ZIC 1

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Citation for Human/Mouse ZIC1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A transcriptome atlas of the mouse iris at single-cell resolution defines cell types and the genomic response to pupil dilation
    Authors: J Wang, A Rattner, J Nathans
    Elife, 2021;10(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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