Human Mrc2 Antibody

Detection of Human Mrc2 by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Mrc2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5770) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Mrc2 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Mrc2 in Human Prostate Cancer Tissue.

Mrc2 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human Mrc2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5770) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Mrc2 by Western Blot

SEL1L, but not HRD1, positively regulates MRC2.a Flow-cytometry based collagen-uptake assay in HEK293T of different genotypes (compared with baseline control without fluorescent collagen). N = 4 (Baseline), 5 (WT, SEL1L-KO, HRD1-KO) per group; p = 0.0209 (vs. SEL1L-KO), p = 0.7682 (vs. HRD1-KO). b, c Western blot and densitometry of HEK293T cells of different genotypes, representative of N = 5 independent experiments; p = 0.0120 (WT vs. SEL1L-KO), p = 0.5143 (WT vs. HRD1-KO). d Firefly luciferase assay of MRC2 promoter activity, normalized to constitutive Renilla luciferase in HEK293T cells of different genotypes. N = 3 per group; p = 0.011 (WT vs. SEL1L-KO), p = 0.0162 (SEL1L-KO vs. HRD1-KO), p = 0.078 (WT vs. HRD1-KO). e Western blot of HEK293T cells of different genotypes transfected with MRC2 overexpression vector vs. vehicle control, representative of N = 6 independent experiments. f Representative wide-field immunofluorescence images of HEK293T cells of different genotypes transfected with MRC2 overexpression vector vs. vehicle control. g Flow-cytometry based collagen-uptake assay in HEK293T of different genotypes transfected with MRC2 overexpression vector (compared with baseline control without fluorescent collagen; note different scale compared with panel a). N = 3 (Baseline), 4 (WT, SEL1L-KO, HRD1-KO) per group; p = 0.9993 (WT vs. SEL1L-KO), p = 0.9898 (SEL1L vs. HRD1-KO). Data are shown as the mean ± SEM. Statistics: a, g one-way ANOVA with post-hoc Tukey testing; c repeated-measures ANOVA with post-hoc Bonferroni testing; d one-way ANOVA with post-hoc Bonferroni testing. *p < 0.05, **p < 0.01, ns not significant. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41467-024-45817-8), licensed under a CC-BY license. Not internally tested by R&D Systems.