Human Mrc2 Antibody

Catalog # Availability Size / Price Qty
AF5770
AF5770-SP
Detection of Human Mrc2 by Western Blot.
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Human Mrc2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Mrc2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant mouse Mrc2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Mrc2
Cys800-Ala1059
Accession # Q9UBG0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Mrc2 antibody by Western Blot. View Larger

Detection of Human Mrc2 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Mrc2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5770) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Mrc2 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry Mrc2 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Mrc2 in Human Prostate Cancer Tissue. Mrc2 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human Mrc2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5770) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mrc2

Mrc2 (C-type Mannose Receptor 2; also MMR2, Endocytic Receptor 180 and uPARAP) is a 180‑200 kDa member of the macrophage mannose receptor family of proteins. It is widely expressed, being found on fibroblasts, monocytes/macrophages, endothelial cells, keratinocytes and myoepithelial cells. It binds the carbohydrates Man, Fuc and NAcGlc, type V collagen, and pro‑uPA. Mature human Mrc2 is a 1449 amino acid (aa) type I transmembrane protein. It contains a 1384 aa extracellular region and a 44 aa cytoplasmic domain. The extracellular region shows one ricin B‑type lectin domain (aa 41‑167), one fibronectin type II domain (aa 182‑230) and eight C‑type lectin domains (aa 244‑1393). Over aa 800‑1059, human Mrc2 shares 87% aa identity with mouse Mrc2.

Long Name
Mannose Receptor, C-type 2
Entrez Gene IDs
9902 (Human); 17534 (Mouse); 498011 (Rat)
Alternate Names
CD280 antigen; CD280; CD280C-type mannose receptor 2; CLEC13E; CLEC13EUPAR-associated protein; Endo180; ENDO180C-type lectin domain family 13 member E; Endocytic receptor 180; KIAA0709FLJ35911; Macrophage mannose receptor 2; mannose receptor, C type 2; Mrc2; uPARAP; UPARAPendocytic receptor (macrophage mannose receptor family); urokinase plasminogen activator receptor-associated protein; Urokinase receptor-associated protein; Urokinase-type plasminogen activator receptor-associated protein

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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