Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 358621
View all Myeloperoxidase/MPO Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived human Myeloperoxidase/MPO protein
Ala49-Ser745
Accession # P05164

Specificity

Detects human Myeloperoxidase/MPO in sandwich ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human Myeloperoxidase/MPO Antibody

Human Myeloperoxidase/MPO ELISA Standard Curve.

Recombinant Human Myeloperoxidase/MPO protein was serially diluted 2-fold and captured by Rat Anti-Human Myeloperoxidase/MPO Monoclonal Antibody (Catalog # MAB31741) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Applications for Human Myeloperoxidase/MPO Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody.

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Myeloperoxidase DuoSet ELISA Kit (Catalog # DY3174) for convenient development of a sandwich ELISA or the Human Myeloperoxidase Quantikine ELISA Kit (Catalog # DMYE00B) for  a complete optimized ELISA.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Myeloperoxidase/MPO

Myeloperoxidase (MPO) is a hemeprotein that belongs to the XPO subfamily of the heme peroxidase superfamily. MPO is synthesized as a preproprotein that undergoes proteolytic processing to generate a disulfide-linked heterodimer of the N-terminal beta -subunit (12 kDa) and C-terminal alpha subunit (60 kDa). Active MPO is a tetramer of two beta -subunits and two alpha -subunits that are also disulfide-linked through the two alpha -subunits. MPO is stored in granules and is an abundant protein in neutrophils and monocytes. MPO is released upon activation to catalyze the formation of powerful oxidants such as hypochlorous acid, which kills microbes. Unprocessed pro-MPO can also be released. Human and mouse MPO share 87% amino acid sequence identity.

Alternate Names

MPO

Entrez Gene IDs

4353 (Human); 17523 (Mouse); 303413 (Rat)

Gene Symbol

MPO

UniProt

P05164

Additional Myeloperoxidase/MPO Products

Product Documents for Human Myeloperoxidase/MPO Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Myeloperoxidase/MPO Antibody

For research use only

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