Detection of Human NDRG1 by Western Blot.
Western blot shows lysates of human liver tissue and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human NDRG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5209) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NDRG1 at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
NDRG1 (N-myc downstream-regulated gene 1; also DRG-1 and Cap43) is a 43 kDa member of the NDRG family of proteins. It is ubiquitously expressed, found in fibroblasts, endothelial cells, respiratory epithelium and prostate epithelium. NDRG1 is highly expressed in nonproliferating, differentiating tissue. Human NDRG1 is 394 amino acids (aa) in length. It contains an NDGR domain (aa 286‑316) plus three tandem 10 aa hydrophilic repeats (aa 339‑368). There are three potential isoform variants. Two involve alternate start sites at Met82 and Met286, while a third shows a four aa substitution for aa 1‑34. Over aa 11‑267, human NDRG1 is 95% aa identical to mouse NDRG1.
N-myc Downstream Regulated Gene 1
Entrez Gene IDs:
10397 (Human); 17988 (Mouse); 299923 (Rat)
CAP43; CMT4D; Differentiation-related gene 1 protein; DRG1; DRG-1; DRG1HMSNL; GC4; HMSNL; NDR1; NDRG1; Nickel-specific induction protein Cap43; NMSL; N-myc downstream regulated 1; N-myc downstream-regulated gene 1 protein; protein NDRG1; protein regulated by oxygen-1; PROXY1; Reducing agents and tunicamycin-responsive protein; RIT42; RTP; RTPGC4; TARG1; TDD5; tunicamycin-responsive protein
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