|Detection of Human NEDD9 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human NEDD9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5459) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for NEDD9 at approximately 105 and 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.|
NEDD9 (also HEF1 and Cas-L) is a 93 kDa (predicted) member of the CAS family of proteins. It is widely expressed, and serves to integrate integrin beta 1 and BCR signaling with cell mitotic pathways. Human NEDD9 is 834 amino acids (aa) in length and contains one SH3 domain (aa 3‑65), a Tyr-rich SH2-binding region (aa 90‑350), one Ser-rich four alpha -helix bundle protein interaction site (aa 350‑650), and an HLH motif that mediates Cas protein dimerization (aa 710‑760). NEDD9 usually runs at 105 or 115 kDa in SDS‑PAGE due to phosphorylation. It is also cleaved near Leu361 to generate an active N-terminal 55 kDa fragment. One isoform shows a deletion of aa 155‑188, while two others show 20 and seven aa substitutions for aa 155‑834 and 1‑153, respectively. Over aa 153‑366, human and mouse NEDD9 share 85% aa identity.
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