Human Netrin-4 Antibody
Human Netrin-4 Antibody Summary
Val26-Lys628
Accession # Q9HB63
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Netrin-4
Netrins/UNC-6 (netr: Sanskrit for “one who guides”) are a family of laminin-related small proteins that are involved in neurite outgrowth and axon guidance. Netrins bind to the DCC and UNC5 family of receptors to attract or repel axons. Human Netrin-4 is synthesized as a 628 amino acid (aa) precursor that contains a 19 aa signal sequence, a 443 aa laminin-related region containing an N-terminal laminin globular domain (domain VI) followed by 3 laminin EGF-like repeats, and a 166 aa C‑terminal domain rich in basic aa residues that serves as a heparin binding site. Unlike human Netrin-1 which is reminiscent of the laminin gamma -chain, human Netrin-4 resembles the laminin beta -chain. Netrin-4 has been reported to exist as both a monomer and a dimer. The dimeric form was reported to be the less active of the two. Human Netrin-4 shares 31%, 29% and 25% aa sequence identity with human Netrin-1, 2 and G2, respectively; it shares 89% aa identity with mouse Netrin-4. Netrin-4 has widespread expression, occurring in nervous tissues such as embryonic floor plate and postnatal neurons such as cerebellar granule cells and hippocampal pyramidal cells. It also is found in non-neural tissues, such as adult Bowman’s capsule and medullary tubular epithelium in kidney, and embryonic pancreatic and intestinal epithelium, plus cells of the ureteric bud. Netrin-4 expression is often associated with basement membrane. Netrin-4 has been shown to initiate axon outgrowth from olfactory bulb explants (1‑7).
- McFarlane, S. (2000) Biochem. Cell. Biol. 78:563.
- Kennedy, T.E. (2000) Biochem. Cell Biol. 78:569.
- Yu, T.W. and C.I. Bargmann (2001) Nature Neurosci. 4(Suppl):1169.
- Stein, E. et al. (2001) Science 291:1976.
- Koch, M. et al. (2000) J. Cell Biol. 151:221.
- Yin, Y. et al. (2000) Mech. Dev. 96:115.
- Livesey, F.J. (1999) Cell. Mol. Life Sci. 56:62.
Product Datasheets
Product Specific Notices
This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.U.S. Patent # 5,824,775, 6,218,526, and other U.S. and international patents pending.
Citations for Human Netrin-4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Dorsal horn interneuron-derived Netrin-4 contributes to spinal sensitization in chronic pain via Unc5B
Authors: Toshihide Yamashita
J. Exp. Med., 2016;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Identification of NTN4, TRA1, and STC2 as prognostic markers in breast cancer in a screen for signal sequence encoding proteins.
Authors: Esseghir S, Kennedy A, Seedhar P, Nerurkar A, Poulsom R, Reis-Filho JS, Isacke CM
Clin. Cancer Res., 2007;13(11):3164-73.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr
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