Human Neurogenin-1 Antibody (309707)

R&D Systems | Catalog # MAB3524

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 309707
View all Neurogenin-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Neurogenin‑1
Ala145-His237
Accession # NP_006152

Specificity

Detects human Neurogenin-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Neurogenin-3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human Neurogenin-1 Antibody (309707)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Neurogenin‑1

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Neurogenin-1

Neurogenin-1 is a 26 kDa developmentally expressed nuclear protein of the basic helix-loop-helix (bHLH) family, class B subfamily. bHLH proteins are transcription factors that form dimers to bind DNA. NGN1 is expressed only in embryonic neural tissue and supports neuronal differentiation but inhibits glial cell differentiation. Human Neurogenin-1 shows 93% amino acid identity with both mouse and rat Neurogenin-1 over the sequence used as an immunogen.

Alternate Names

AKA, Math4C, NEUROD3, NEUROG1, Neurogenin1

Entrez Gene IDs

4762 (Human); 18014 (Mouse)

Gene Symbol

NEUROG1

UniProt

NP_006152

Additional Neurogenin-1 Products

Product Documents for Human Neurogenin-1 Antibody (309707)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Neurogenin-1 Antibody (309707)

For research use only

Citations for Human Neurogenin-1 Antibody (309707)

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