Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all NF-M Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NF-M
Ser2-Ser358
Accession # P07197

Specificity

Detects human NF-M in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human (rh) NF-H and rhNF-L is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human NF‑M Antibody

Western Blot Shows Human NF-M Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human NF‑M Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NF-M knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human NF-M Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3029) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NF-M at approximately 155 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human NF‑M Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum and cortex)

Knockout Validated

NF‑M is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in NF‑M knockout HeLa cell line.

Western Blot

NF‑M is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in NF‑M knockout HeLa cell line.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: NF-M

Human NF-M (neurofilament-medium length) is a 145‑150 kDa phosphorylated glycoprotein that belongs to the type IV intermediate filament family. It is 915 amino acids in length and contains a 100 amino acid (aa) N-terminal globular region, a 320 aa alpha -helical rod, or central region, and a 500 aa globular tail. The rod region contains a series of heptad repeats that contribute to a coiled-coil interaction with an adjacent NF-M molecule, the first step in intermediate filament oligomerization. The C-terminus shows phosphorylation and O-linked glycosylation. Human NF-M aa 2‑358, which includes most of the rod region, shares ~98% aa sequence identity with corresponding region of mouse, rat and canine NF-M.

Long Name

Neurofilament Protein, Medium Chain

Alternate Names

NEF3, NEFM, NFM

Entrez Gene IDs

4741 (Human)

Gene Symbol

NEFM

UniProt

P07197

Additional NF-M Products

Product Documents for Human NF‑M Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human NF‑M Antibody

For research use only

Citations for Human NF‑M Antibody

Customer Reviews for Human NF‑M Antibody

There are currently no reviews for this product. Be the first to review Human NF‑M Antibody and earn rewards!

Have you used Human NF‑M Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies