Human NGFI‑B alpha /Nur77/NR4A1 Antibody

R&D Systems | Catalog # PP-H1648-00

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Direct ELISA, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # H1648
View all NGFI-B alpha/Nur77/NR4A1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human NGFI-B alpha/Nur77/NR4A1
aa 2-249

Specificity

This antibody specifically recognizes human NGFI-B alpha but does not recognize NGFI-B beta and gamma. Not yet tested in other species.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human NGFI‑B alpha /Nur77/NR4A1 Antibody

Application
Recommended Usage

Direct ELISA

This antibody can be used at 0.3 μg/mL with the appropriate secondary reagents to detect human NGFI-B alpha.

Immunoprecipitation

Optimal dilutions should be determined by each laboratory.

Western Blot

This antibody can be used at 1 μg/mL with the appropriate secondary reagents to detect human NGFI-B alpha.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

A liquid formulation in physiologic saline with 0.1% NaN3.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: NGFI-B alpha/Nur77/NR4A1

Nerve growth factor inducible factor I-B alpha (NGFI-B alpha, Nur77, TR3, HMR; NR4A1) is a member of the Orphan Nuclear Receptor family. NGFI-B alpha is expressed in the brain, adrenals, thyroid, liver, testis, ovary, thymus, muscle, lung and prostate. Its expression is induced in response to various stress stimuli and growth factors. NGFI-B alpha is involved in mediating apoptotic signaling in thymocytes and tumor cells, as well as having a role in signaling in the hypothalamic-pituitary axis. RXR has been shown to be a partner for NGFI-B alpha.

Long Name

Nerve Growth Factor-induced Protein I-B alpha/Nuclear Receptor Subfamily 4, Group A, Member 1

Alternate Names

HMR, NAK1, NGFIB alpha, NR4A1, Nur77, TR3

Entrez Gene IDs

3164 (Human)

Gene Symbol

NR4A1

Additional NGFI-B alpha/Nur77/NR4A1 Products

Product Documents for Human NGFI‑B alpha /Nur77/NR4A1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human NGFI‑B alpha /Nur77/NR4A1 Antibody

For research use only

Citations for Human NGFI‑B alpha /Nur77/NR4A1 Antibody

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