Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat

Applications

Validated:

Western Blot

Cited:

Western Blot, Flow Cytometry, Slot Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all NgR2/NgRH1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human NgR2/NgRH1
Ala28-Leu401
Accession # Q86UN3

Specificity

Detects human NgR2/NgRH1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) NgR and rhNgRH2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human NgR2/NgRH1 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human NgR2/NgRH1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NgR2/NgRH1

NgR2, also known as reticulon 4 receptor-like 2 and NgR2, is a 60 kDa glycosylphosphatidylinositol-anchored protein that is expressed primarily in the brain. It contains eight leucine-rich repeats that are flanked by cysteine-rich sequences at both the N- as well as the C-terminus. Membrane-anchored NgR2 can be solubilized through the action of phospholipase or an unidentified MT-MMP to generate a 46 kDa soluble receptor. Mature human NgR2 shares 48% aa sequence identity with human NgRH2. It also shares 96% aa sequence identity with mouse and rat mature NgR2.

Long Name

Nogo-66 Receptor-related Protein 2/Nogo-66 Receptor Homolog 1/Reticulon-4 Receptor-like 2

Alternate Names

NgRH1, RTN4RL2

Entrez Gene IDs

349667 (Human); 269295 (Mouse)

Gene Symbol

RTN4RL2

UniProt

Q86UN3

Additional NgR2/NgRH1 Products

Product Documents for Human NgR2/NgRH1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human NgR2/NgRH1 Antibody

For research use only

Citations for Human NgR2/NgRH1 Antibody

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