Human NgR2/NgRH1 Antibody

Catalog # Availability Size / Price Qty
AF2776
AF2776-SP
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Human NgR2/NgRH1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human NgR2/NgRH1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) NgR and rhNgRH2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human NgR2/NgRH1
Ala28-Leu401
Accession # Q86UN3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human NgR2/NgRH1

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NgR2/NgRH1

NgR2, also known as reticulon 4 receptor-like 2 and NgR2, is a 60 kDa glycosylphosphatidylinositol-anchored protein that is expressed primarily in the brain. It contains eight leucine-rich repeats that are flanked by cysteine-rich sequences at both the N- as well as the C-terminus. Membrane-anchored NgR2 can be solubilized through the action of phospholipase or an unidentified MT-MMP to generate a 46 kDa soluble receptor. Mature human NgR2 shares 48% aa sequence identity with human NgRH2. It also shares 96% aa sequence identity with mouse and rat mature NgR2.

Long Name
Nogo-66 Receptor-related Protein 2/Nogo-66 Receptor Homolog 1/Reticulon-4 Receptor-like 2
Entrez Gene IDs
349667 (Human); 269295 (Mouse)
Alternate Names
NgR2; NgRH1; reticulon 4 receptor-like 2; RTN4RL2

Product Datasheets

Citations for Human NgR2/NgRH1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Ganglioside inhibition of neurite outgrowth requires Nogo receptor function: identification of interaction sites and development of novel antagonists.
    Authors: Williams G, Wood A, Williams EJ, Gao Y, Mercado ML, Katz A, Joseph-McCarthy D, Bates B, Ling HP, Aulabaugh A, Zaccardi J, Xie Y, Pangalos MN, Walsh FS, Doherty P
    J. Biol. Chem., 2008;283(24):16641-52.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. A role for Nogo receptor in macrophage clearance from injured peripheral nerve.
    Authors: Fry EJ, Ho C, David S
    Neuron, 2007;53(5):649-62.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Slot Blot

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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