Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all NgR3/NgRH2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human NgR3/NgRH2
Cys25-Ala419
Accession # Q86UN2

Specificity

Detects human NgR3/NgRH2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross‑reactivity with recombinant human (rh) NgRH1 is observed and less than 1% cross-reactivity with rhNgR is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human NgR3/NgRH2 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human NgR3/NgRH2

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NgR3/NgRH2

NgR3, also known as Nogo 66 receptor homolog 2 (NgRH2) and reticulon 4 receptor-like 1 (RTN4RL1), is a 60 kDa GPI-linked glycoprotein belonging to the family of leucine-rich repeat (LRR) proteins. It contains eight central LRRs that are flanked by a LRR N-terminal subdomain and a cysteine-rich C-terminal subdomain. Human NgR3 shares approximately 89% amino acid sequence homology with the mouse or rat protein.

Long Name

Nogo-66 Receptor-related Protein 3/Nogo-66 Receptor Homolog 2/Reticulon-4 Receptor-like 1

Alternate Names

NgRH2, RTN4RL1

Entrez Gene IDs

146760 (Human); 237847 (Mouse)

Gene Symbol

RTN4RL1

UniProt

Q86UN2

Additional NgR3/NgRH2 Products

Product Documents for Human NgR3/NgRH2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human NgR3/NgRH2 Antibody

For research use only

Related Research Areas

Glial Lineage Markers

Citations for Human NgR3/NgRH2 Antibody

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