Human Nicastrin Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human Nicastrin in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 716910
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human Nicastrin
    Asn34-Glu669
    Accession # Q92542
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Human Nicastrin by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Nicastrin Monoclonal Antibody (Catalog # MAB53781) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Nicastrin at approximately 110-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nicastrin

Nicastrin (NCT) is a 150‑160 kDa member of the nicastrin family of proteins. It is a component of the aspartyl protease gamma -secretase complex and serves to stabilize and direct gamma -secretase components to proper positions in the plasma membrane. The gamma -secretase complex mediates the cleavage of intramembrane proteins such as notch-1 and APP. Mature human nicastrin is a 676 amino acid type I transmembrane glycoprotein. It contains a 636 aa extracellular domain (aa 34‑669) that shows a 58 aa sequence (aa 312‑369) which interacts with gamma -secretase substrates. There are multiple splice variants of NCT. One shows a deletion of aa 195‑322 and 394‑709, a second shows a 29 aa substitution for the C-terminal 604 aa and a third shows a deletion of aa 200‑709 accompanied by an insertion of 33 aa after Leu30. Over aa 34‑669, human NCT shares 90% aa identity with mouse NCT.

  • Entrez Gene IDs:
    23385 (Human); 59287 (Mouse); 289231 (Rat)
  • Alternate Names:
    anterior pharynx-defective 2; APH2; ATAG1874; KIAA0253APH2; NCSTN; Nicastrin
Related Research Areas

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 148  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 32  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 12  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow , IHC , ICC NL007 10
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 4  
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG Antibody

WB AF007 4
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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