Detection of Human NOMO by Western Blot.
Western blot shows lysates of LNCaP human prostate cancer cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human NOMO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3755) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NOMO at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
NOMO, also known as pM5, is a 130 kDa type I membrane protein with no
recognizable functional motif. It is a binding partner of Nicalin, a
nicastin-related protein. The Nicalin/NOMO complex is localized in the
ER and functions as an antagonist for Nodal and Activin signaling.
Three copies of the NOMO gene, encoding NOMO isoforms 1, 2 and 3 have
been described. The extracellular domains (ECD) of the isoforms share
99% amino acid sequence homology. The ECD of human and mouse NOMO also
share 94% amino acid sequence homology.
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