Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Applications

Validated:

Western Blot

Cited:

Western Blot, ELISA Capture, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 209808
View all NOV/CCN3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human NOV/CCN3
Thr32-Met357
Accession # P48745

Specificity

Detects human NOV/CCN3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant mouse NOV, recombinant human (rh) CTGF, or rhWISP-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human NOV/CCN3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human NOV/CCN3 (Catalog # 1640-NV)
under non-reducing conditions only

Reviewed Applications

Read 4 reviews rated 3.8 using MAB1640 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NOV/CCN3

NOV, also called CCN3, is one of six CCN (CYR61/CTGF/NOV) secreted proteins which share a common multimodular organization (1 - 4). NOV/CCN3 contains an N-terminal IGFBP domain that appears to be non-functional and a vWF type C and thrombospondin type I domain which mediate oligomerization and matrix interactions, respectively (1, 2). The C-terminal cysteine knot domain interacts with several partners, including the matrix protein fibulin 1C (5), Notch-1 (6), and CCN2, with which it may heterodimerize (2). NOV/CCN3 also interacts with the gap junction protein Connexin43 and mediates suppression of proliferation (7). It also binds the calcium binding protein S100A4 and promotes calcium channel activation (8). The 357 amino acid (aa), 44 kDa human NOV/CCN3 shares 80% aa identity with mouse, rat and dog NOV/CCN3, and 78% aa identity with cow NOV/CCN3. NOV/CCN3 also shows 38 - 50% aa identity with other family members including WISP proteins, except for WISP-2/CCN5 which lacks the cysteine knot (1). NOV/CCN3 is widely expressed developmentally, especially in muscle, endothelium, nervous system, adrenal cortex and chondrocytes (1 - 4). In transformed cells, a 32 kDa N-terminally truncated form lacks the signal sequence is localized to the nucleus. Truncation allows a C-terminal nuclear localization sequence to be active (9). Nuclear NOV/CCN3 acts as a transcriptional repressor but promotes proliferation, presumably by interfering with growth control (9). Full length NOV/CCN3 is a secreted matricellular protein which inhibits cell growth. Interaction of NOV/CCN3 with integrins alpha v beta 3 and  alpha 5 beta 1 mediates endothelial cell adhesion, chemotaxis, and promotes angiogenesis (10, 11). Over-expression of NOV/CCN3 downregulates myogenic genes such as MyoD (12).

References

  1. Perbal, B. (2004) Lancet 363:62.
  2. Perbal, B. (2006) Cell Commun. Signal. 4:3.
  3. Martinerie, C. et al. (1994) Oncogene 9:2729.
  4. Snaith, M.R. et al. (1996) Genomics 38:425.
  5. Perbal, B. et al. (1999) Proc. Natl. Acad. Sci. USA 96:869.
  6. Sakamoto, K. et al. (2002) J. Biol. Chem. 277:29399.
  7. Fu, C.T. et al. (2004) J. Biol. Chem. 279:36943.
  8. Li, C.L. et al. (2002) Mol. Pathol. 55:250.
  9. Planque, N. et al. (2006) J. Cell. Biochem. 99:105.
  10. Lin, C.G. et al. (2003) J. Biol. Chem. 278:24200.
  11. Lin, C.G. et al. (2003) J. Biol. Chem. 280:8229.
  12. Calhabeu, F. et al. (2006) Exp. Cell. Res. 312:1876.

Long Name

Nephroblastoma Overexpressed Gene

Alternate Names

CCN3, IGFBP-9

Entrez Gene IDs

4856 (Human); 18133 (Mouse)

Gene Symbol

CCN3

UniProt

P48745

Additional NOV/CCN3 Products

Product Documents for Human NOV/CCN3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NOV/CCN3 Antibody

For research use only

Citations for Human NOV/CCN3 Antibody

Customer Reviews for Human NOV/CCN3 Antibody (4)

3.8 out of 5
4 Customer Ratings
5 Stars
0%
4 Stars
75%
3 Stars
25%
2 Stars
0%
1 Stars
0%

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  • Human NOV/CCN3 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    Human NOV/CCN3 Antibody MAB1640
  • Human NOV/CCN3 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/14/2018
  • Human NOV/CCN3 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Plasma
    Species: Human
    Verified Customer | Posted 11/10/2018
  • Human NOV/CCN3 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 10/09/2018

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FAQs

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail