Detection of Human NPDC‑1 by Western Blot.
Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human NPDC‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5489) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for NPDC‑1 at approximately 40 kDa and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
NPDC‑1 in Human Brain.
NPDC‑1 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Sheep Anti-Human NPDC‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5489) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and synaptic vesicles of neuronal processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
NPDC-1 (Neural proliferation differentiation and control protein 1) is a 35 kDa member of the NPDC1/cab1 family of proteins. It is principally expressed in neurons, and serves as an anti-proliferation agent. It may accomplish this, in part, by binding to E2F-1 and blocking its protranscriptional activity. Mature human NPDC-1 is 291 amino acids (aa) in length. It is a type I transmembrane protein that contains an NLS (aa 107‑124), an HLH domain (aa 95‑128), a transmembrane domain (aa 182‑202), and a PEST degradation sequence (aa 269‑302). Phosphorylation promotes ubiquitination at this latter site. There are two splice variants. One shows a deletion of aa 242‑263, while another shows a nine aa substitution for the nine aa in positions 218‑226. Over aa 35‑181, human NPDC-1 shares 68% aa identity with mouse NPDC-1.
Neural Proliferation, Differentiation and Control, 1
Entrez Gene IDs:
56654 (Human); 18146 (Mouse); 296562 (Rat)
CAB-; CAB1; CAB-1; CAB1CAB; DKFZp586J0523; neural proliferation differentiation and control protein 1; neural proliferation, differentiation and control, 1; NPDC1; NPDC-1
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