|Detection of Human NPDC‑1 by Western Blot. Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human NPDC‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5489) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for NPDC‑1 at approximately 40 kDa and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.|
|NPDC‑1 in Human Brain. NPDC‑1 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Sheep Anti-Human NPDC‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5489) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and synaptic vesicles of neuronal processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.|
NPDC-1 (Neural proliferation differentiation and control protein 1) is a 35 kDa member of the NPDC1/cab1 family of proteins. It is principally expressed in neurons, and serves as an anti-proliferation agent. It may accomplish this, in part, by binding to E2F-1 and blocking its protranscriptional activity. Mature human NPDC-1 is 291 amino acids (aa) in length. It is a type I transmembrane protein that contains an NLS (aa 107‑124), an HLH domain (aa 95‑128), a transmembrane domain (aa 182‑202), and a PEST degradation sequence (aa 269‑302). Phosphorylation promotes ubiquitination at this latter site. There are two splice variants. One shows a deletion of aa 242‑263, while another shows a nine aa substitution for the nine aa in positions 218‑226. Over aa 35‑181, human NPDC-1 shares 68% aa identity with mouse NPDC-1.