Human NRG1-beta 1/HRG1-beta 1 EGF Domain Antibody

Catalog # Availability Size / Price Qty
MAB3771-SP
MAB3771-500
MAB3771-100
Product Details
Citations (2)
FAQs
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Human NRG1-beta 1/HRG1-beta 1 EGF Domain Antibody Summary

Species Reactivity
Human
Specificity
Detects human NRG1-beta 1 in ELISAs. Recognizes an epitope found in the EGF-like domain of NRG1‑ beta 1 (amino acids 179‑246). It also detects recombinant human SMDF (Sensory and Motor neuron-Derived Factor), but it does not recognize the EGF-like domain of human NRG1-alpha (amino acids 177‑241). The epitope is present within amino acids 213‑230 of human NRG1-beta 1. This segment is present in all NRG1 beta isoforms, GGF‑2 (glial growth factor), and SMDF, but it is absent from NRG1-alpha, NDF43, and NRG1-gamma.
Source
Monoclonal Mouse IgG1 Clone # 147705
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human NRG1-beta 1/HRG1-beta 1
Ser2-Lys246
Accession # AAA58639
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample

Human NRG1-beta 1/HRG1-beta 1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human NRG1‑ beta 1/HRG1‑ beta 1 Extracellular Domain Biotinylated Antibody (Catalog # BAF377)

Standard: Recombinant Human NRG1-beta 1/HRG1-beta 1 ECD Protein (Catalog # 377-HB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuregulin-1 beta 1/NRG1 beta 1

Neuregulin-1, also known as Heregulin 1, functions as a ligand for the receptor tyrosine kinases, ErbB3 and ErbB4. It exists in several different mRNA splicing variants resulting in both transmembrane and secreted forms.

Entrez Gene IDs
3084 (Human); 211323 (Mouse); 112400 (Rat)
Alternate Names
ARIA; GGF; GGF2; HGL; HRG; HRG1; HRGA; MST131; MSTP131; NDF; neuregulin 1; Neuregulin1 beta 1; Neuregulin-1 beta 1; NRG1-IT2; SMDF

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Citations for Human NRG1-beta 1/HRG1-beta 1 EGF Domain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Activation of the neuregulin/ErbB system during physiological ventricular remodeling in pregnancy.
    Authors: Lemmens K, Doggen K, De Keulenaer GW
    Am. J. Physiol. Heart Circ. Physiol., 2011;300(3):H931-42.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells.
    Authors: Sheng, Qing, Liu, Xinggang, Fleming, Eleanor, Yuan, Karen, Piao, Huiying, Chen, Jinyun, Moustafa, Zeinab, Thomas, Roman K, Greulich, Heidi, Schinzel, Anna, Zaghlul, Sara, Batt, David, Ettenberg, Seth, Meyerson, Matthew, Schoeberl, Birgit, Kung, Andrew L, Hahn, William, Drapkin, Ronny, Livingston, David M, Liu, Joyce F
    Cancer Cell, 2010;17(3):298-310.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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