Detects human NRG1-beta 1 in ELISAs and Western blots. Recognizes an epitope found in the EGF-like domain of NRG1‑ beta 1 (amino acids 179‑246). It also detects recombinant human SMDF (Sensory and Motor neuron-Derived Factor), but it does not recognize the EGF-like domain of human NRG1-alpha (amino acids 177‑241). The epitope is present within amino acids 213‑230 of human NRG1-beta 1. This segment is present in all NRG1 beta isoforms, GGF‑2 (glial growth factor), and SMDF, but it is absent from NRG1-alpha, NDF43, and NRG1-gamma.
Monoclonal Mouse IgG1 Clone # 147705
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human NRG1-beta 1/HRG1-beta 1 Ser2-Lys246 Accession # AAA58639
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Human NRG1-beta 1/HRG1-beta 1 Sandwich Immunoassay
ELISA Capture (Matched Antibody Pair)
Human NRG1-beta 1/HRG1-beta 1 EGF Domain Antibody (Catalog # MAB3771)
Detection of Human NRG1‑ beta 1/HRG1‑ beta 1 by Western Blot. Western blot shows lysates of Bowes human melanoma cell line. PVDF membrane was probed with 2 µg/mL of Human NRG1-beta 1/HRG1-beta 1 Monoclonal Antibody (Catalog # MAB3771) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NRG1‑ beta 1/HRG1‑ beta 1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neuregulin-1 beta 1/NRG1 beta 1
Neuregulin-1, also known as Heregulin 1, functions as a ligand for the receptor tyrosine kinases, ErbB3 and ErbB4. It exists in several different mRNA splicing variants resulting in both transmembrane and secreted forms.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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